.1, The Role Of Estrogen On Vascular Endothelial Cells For Buerger Disease, The Role Of Nf-kappab In Atherosclerosis Research

Objective To investigate the alteration of estrogen level in Buerger disease patients and the effect of varied estrogen level in Buerger disease vascular endothelial cells.Methods Two groups of arteria vascular endothelial cells (one group deriving from the male Buerger disease patients; the other group deriving from the normal men) are detected ER> NF-kappa BP65 protein', ICAM-1', VCAM-1', CD59 and IgG expressions by immunohistochemistry staining.Results (1) The expression of ER> NF-kappa BP65 protein', ICAM-1',VCAM-1'. and CD59 of the male Buerger disease patients' arteria vascular endothelial cells is much more reinforced than that of the normal men(P< 0.001); The expression of IgG of the male Buerger disease patients'arteria vascular endothelial cells is much more feebly than that of the normal men(P< 0.001). Although the expression of IgG of under arteria intima the male Buerger disease patients is much more reinforced than that of the normal men(P< 0.001). (2) The detected data from all the arteria vascular endothelial cells illuminates that ER's and NF-kappa BP65 protein's expressions are coincided in linear regression(b= 0.706, P< 0.001); ER's and IgG's expressions are coincided in linear regression(b= -0.594, P< 0.001); CD59's and IgG's expressions are coincided in linear regression(b= -0.547, P< 0.001); ER's expression of all the arteria vascular endothelial cells and IgG's expression of under arteria intima illuminate that they are coincided in linear regression(b= 0.649, P< 0.001).Conclusion (1) The high estrogen level leadNF-kappa B to be activated and the activated NF-kappa B give ICAM-1 and VCAm-1 to express more strongly in Buerger disease patients than normal male men. The expressions' up-regulation of ICAM-1 and VCAM-1 accelerates the immune injuring of vascular endothelialcells in Buerger disease. (2) The high estrogen level lead IgG expression in vivo to rise up, and then accelerates the immune injuring of vascular endothelial cells in Buerger disease. However, the high expression of CD59 in Buerger disease patients' vascular endothelial cells protects from immune injuring that is caused by IgG.