| PrefaceSince early 1990, due to container of highly efficient anti -tumour activity discovered by the researching group of Japanese indus-try and technique center and Hongqian University, the squid ink has been paid attention more and more. Many researchers have gradually made further studies on the anti - tumour effeetion, mechanism and the function of immunoregulation .Squid ink is a kind of self - protective material when it encounter its enemy. It contains large amount of black coloring matter. The mechanism of anti - tumour of squid ink had become the focus noted by many people. At present, the domestic study mainly concentrated on the field of the immunoregulation of squid ink.The result of Lu changlong et al have shown that squid ink and its extract have the remarkable antitumour activity, but it does not exhibit the direct cytotoxicity to the tumour cells. The antitumour effects- of squid ink is due to the enhancement of immune function, especially, the results of the enhancement of cellular immunity. Squid ink has the activity of enhancing macrophage; it can increase the levels of TNF -a IL -2 IFN - γ in the mouse serum ; it has the promoting function on the killing activity of NK cell and LAK cell; it also has the promo-ting function on NO produce of macrophage.We still know little about the association between IL -4 and the mechanism of anti - tumour of squid ink. As a part of a series of stud-ies, based on previous achievements, studing the effects of squid ink on IL - 4 secretion level of lymphocyte, We hope the results obtained in this paper would be helpful to throw light on the mechanism of and - tumour of squid ink.Materials and MethodsTake 36 BALB/C mice, divide into 6 groups, 6 mice each group, among which the fifth group is the experimental group. In the experimental group each mouse is treated orally with 5% concentration squid ink 0. 5 ml each time, one time per day, the control group is treated with NS by the same method as the experimental one. Assume the lymphocytes of mice at the zero day , second day, forth day, sixth day, eighth day after treated orally with squid ink , use ELISA method to determine IL - 4 level.ResultEffects of Squid Ink on IL - 4 Yield Level in Mouse Lymphocytes Using ELISA method, We determine IL - 4 level in mouse lympho-cytes. The IL - 4 level in the culture of mouse lymphocytes which were treated orally with squid ink after 0 day, 2 day , 4 day, 6 day, 8 day is stable. The experimental group is almost alike to the control groupe.DiscussionAnti - tumor effect of immune system can be achieved throughvaries way. There are specific immune response ( such as CTL, anti-body) , and non -specific immune response (such as NK, LAK, ac-tivated Macrophage, neutrophil) ; there are cellular immune response and humoral immune response. Immune response of host to the tumor cells is a co - effect of both cellular immune response and humoral im-mune response.Previous results of this experiment prove that squid ink can stim-ulate and enhance the activity of macrophage and NK. Anti tumor effect of squid ink is associated with NO generation of macrophage. Squid ink can promote the production of cytokines such as TNF, IL -1, IL - 2 and IFN - γ in mice. Squid ink can induce the cytokine pro-duction of Thl cells and activate the macrophage and NK cell of mice, to promote the immune function and anti - tumor effect.IL -4 has anti - tumor effect and mostly produced by Th2 cells. For further study of the mechanism and associated questions of anti -tumor effect of squid ink, we observed the changes of IL - 4 produc-tion of lymphocytes in mice after oral intake of squid ink. Result shows there is no evident change of IL - 4 production changes on 0 day, 2nd day, 4th day, 6th day, 8th day after intake of squid ink. Experiment group showed no evident difference to the control group.According to the cytokine secreted, CD4+ Th cell is divided into three subtype, ThO, Thl, Th2. IL -1, IL -12, IFN - γ inhibit Th0 from develop... |
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