Shandong Honeysuckle Cultivars Ovo Spend With Chicken Feet Flower Varieties And Quality Evaluation

OBJECTIVE To offer scientific data for quality estimating source research and breeding of the two varieties of Lonicera japonica Thunb. METHODS HPLC PAGE and RAPD were used in comparison of the following aspects: content of main available components . bands of isozyme and gene sequence . Besides the content of amino-acid and extracorporeal bacteriostasis effectiveness were also compared. RESULTS Chlorogenic acid can be separated completely with undesirable components and the content reduces as temperature rises. POD isozyme bands have some difference in quantity and grade, in which , A1 A2, can be regarded as special bands for identifying. OPJ 1 4-CACCCGGATG OP 16- CTGCTTAGGG and 0P20-AAGCGGCCTC can be marked in RAPD which is an effective technique in classification and identification of cultivating varieties. Though growing surroundings are similar , the contents of amino-acids are quite different in the two varieties. The biggest difference exists in hydroxyproline .Both of the two varieties can restraint and effectiveness becomes stronger while adding consistency . POD isozyme analysis. RAPD and bacteriosrasis determination are all firstly used in studies ofLonicerajaponica Thunb in the world.