Reverse Pin Guan Yuan, Sanyinjiao Ovariectomized Rats Hpo Axis And Anti-oxidation Experiments

Peri-menopausal syndrome (PMS) is a series of physical and psychological syndromes, which is caused by ovarian failure and endocrine disorder of women before and after menopause.In recent years, due to aging of the population trends, the increasing social pressure and many other factors, the incidence rate of climacteric syndrome tends to increase.The application of hormone replacement therapy has a history of several decades in modern medicine, and today it is still the first choice for climacteric syndrome. However, in recent years, there are more and more reports on its various side effects. Therefore, to find safe and effective treatment gradually attracts people's attention and become the research hotspot. Acupuncture is a kind of effective therapy for climacteric syndrome with less adverse reactions, and has very good prospects. But the research about acupuncture preventive is very less and the selection of acupoints in clinical is primarily based on subjective judgments of doctors. The application of acupoints is complex, variable, and is lack of objective criteria and evidence.In this experiment, we have observed the influences of the levels of E2,FSH,LH,GnRH and MDA,SOD,GSH-Px by "preventive acupuncture" at Sanyinjiao(SP 6), Guanyuan(RN 4) points in ovariectomized rats. The purpose of this study was to evaluate the regulatory effects of "preventive acupuncture"on HPO axis and oxidation resistance, investigate the specificity of acupoints and provide experimental evidences for acupoints selection and application in clinical practice.Main results are as following:1 In comparison with the normal group, E2 level in serum and organzation of the model group was significantly lower(p<0.01), the levels of FSH,LH and GnRH increased obviously(p<0.01; p<0.01; p<0.01).2 Compared with the model group, E2 level of the "preventive acupuncture" groups increased in different degree, but no obvious difference between groups (p >0.05) FSH LH GnRH level reduced in different degree, but there is no obvious difference between them(p>0.05). 3. Compared with the normal group, model rats, serum MDA level significantly increased (p< 0.01), SOD GSH-PX active significantly reduced (p <0.01; p<0.01; p<0.01)4 Compared with the model group, MDA level of the "preventive acupuncture" groups reduced in different degree, but no obvious difference between them (p>0.05) SOD GSH-Px level increased in different degree, but there is no obvious differencebetween groups (p>0.05)Conclusions:1 The "preventive acupuncture" could increase low estrogen level, inhibit elevated gonadotropin levels in ovariectomized rats and has benign regulation to disorganized HPO axis.2 The "preventive acupuncture" could improve the effect of clearing oxygen radicals, for improving the activity of peroxide decomposition enzymes and inhibit the generation of peroxide.3 The influence without obvious difference to HPO axis and antioxidation between "Sanyinjiao" and "Guanyuan" groups.4 The origination and development of PMS involves in multiple organs, such as the liver, spleen and kidney, and is closely related with the Chong and Ren Meridians. The characteristics of Sanyinjiao, Guanyuan, and the results of this study can suggest that multiple organs, meridians,'both innate and acquired fuctions should be considered all together, and in clinical acupoints election we should pay more attention to crossing acupoints for treatment of this disease.