Ea Baihui Yintang Point On The Impact Of Traumatic Brain Injury In Rats Of Brain Slit2 Mrna

literature review and experimental study are two components of the paper.First Part:Literature reviewThe first review is involved in the clinical and experimental study of the acupuncture and moxibustion on the brain injury; The second review is involved in progresses in research work of the axon guidance factor-Slit family from several aspects such as the discovery, structure, distribution and function; meanwhile, the main function and apply of Slit2 is discussed.Second part:Experimental studyObjective:We studied the electroacupuncture influence to the express of Slit2 in the injuried brain, in order to discuss its mechanism action of acupuncturing governor meridian to the injuried brain.Methods:A modified Feeney's free-fall model was used to build the moderate injuried model of the rat We divided 96 rats into three groups randomly:Blank Group, Model Group, and EA Group. After the hit we use the Neural injury severity score (NSS) to evaluate the neurological function on rats. And at 6h,24h,48h, 6d after injury we took the brain tissues to measure the express of Slit2. Also, total RNA was extracted through RT-PCR..Results:(1)The NSS in Model Group was significant higher at 6h,24h,48h,6d when it was compared with the Blank Group,the statistical datas of P are all less than 0.01; While the NSS in EA Group was significant lower at 6h(P<0.01),24h(P<0.05),48h(P<0.05),6d(P<0.05) when it was compared with the Model Group.(2) Compared with the Blank Group, The Slit2 mRNA content of injury local tissues were increased significantly in EA Group and Model Group at the corresponding times after the brain injury, and the statistical datas have significant difference (P<0.01). Compared with the Model Group, the Slit2 mRNA content of injury local tissues were increased obviously in EA Group, and the statistical datas have obviously difference (P<0.05). Among each time period, the Slit2 RNA content rose at first, then decreased, and it reached the peak in the 48h group.Conclusion:EA has therapeutic effect on the brain injury. It maybe through increasing the Slit2 mRNA content to involve in the progress of neuronal regeneration and tissue repairment, Also EA has protective effect on the brain injury maybe through the Slit's functions of mediate axon outgrowth and neuronal migration, from this neuromal function. improves.