Effect Of Electroacupuncture On The Expression Of Slit2 、HSPGs And SrGAP After Focal Cerebral Infarction In Rats

Objective:(1)To observe the expression of Slit2、HSPGs and SrGAP1 on MCAO rats’ cortex and the effect of acupuncture(EA)intervention on the expression;(2) to study the effect of HSPGs on Slit2-Robo;(3) to study the underlying mechanism of EA in neuroplasticity.Methods:80 male SPrague Dawley(SD) rats were randomized into four groups: Control grouP(10), model group(30), non-acupoint group(10), and EA group(30). Thread-tying method was used in model gro- up,non-acupoint group and EA group to clog arteries and then open up after 1.5 h. On 0day、 7days and 14 days, Nissl staining method was used to observe the morphology change of tissue around the infarction. Indirect immunofluorescence staining and(IIF) and Western Blotting(WB)are used to detect expression of the Slit2、HSPGs and SrGAP1 in the ischemic brain.Results: The analysis results of Nissl’s staining, I and WB were basically consistent.(1)mNSS shows that the score of control group was 0 which was lower than the score of EA group, the score of EA group was lower than the non-acupoint group and the score of Model group was the highest. The results of comparison between each two groups( Control group, Model group, non-acupoint group, and EA group) shows statisti- cal difference(P < 0.05). In comparison with the model group, the neurological score had no obvious difference on day 1 and was significant lower in EA grouP on day 7 and day 14(P < 0.05).(2)Nissl’s staining shows that in EA group Nissl bodies increased in alignment; in non-acupoint group Nissl bodies increased and its arrangement was disordered; in Model group Nissl bodies decreased and its arrangement was disordered with edema in the brain. In comparison with the model group, EA group had no obvious difference and shared something which were reduced nissl number, disordered array and brain edema on day 1; even though the EA group was still disordered but the nissl number increased obviously on day 7; on day 14 the EA group was neat arrayed with an increased nissl number.(3) IF and WB(800 Fluorescence microscope) shows that fluorescence degree of Slit2、HSPGs in non-acu Point group was lower than it in model group(P < 0.01), while the fluorescence degree and expression of protein in non-acupoint group were lower than comParison group with statistical significance(P < 0.01), the fluorescence degree and expression of protein in model group were higher than comparison group with statistical significance(P < 0.05). the fluorescence intensity and grey level of rat Slit2 and SrGAP1 has no obvious difference between model group and EA group on day 1(P > 0.05); but on day 7 and day14, the fluorescence intensity and grey level of EA group was significant higher that model group(P<0.05). Also, along with the change of duration, the fluorescence intensity and grey level were getting higher in both groups.Conclusion :After focal cerebral infarction, the expression of Slit2、HSPGs and SrGAP was low in comParison group, their neurological function and obviously extend the expression time of Slit2、HSPGs and SrGAP after 7days’ electroacPuncture, it had decreased after 14days’ electroacpuncture, which illustrates that EA can reduce the expression of after 14days’ electroacpuncture to improve the recovery of neural function. This could be one of the mechanisms of EA promoting the recovery of neural function after cerebral infarction.