The Impact The The Kidney Tongqiao Side Of The Type ¢ñ Diabetic Rats, The Anterior Hypothalamic Area And Lateral Septal Nucleus Neurons In Bax And Bcl-2 Protein Expression

Objective To investigate the effects of the Bushen Tongqiao Recipe (BS TQR) on expressions of Bax and Bcl-2 in the hypothalamus neurons of I T diabetic rats and to research the therapeutic effect of Bushen Tongqiao Recipe on the degeneration of the hypothalamus neurons of diabetic rats.Methods 40 SD male rats were randomly divided into 4 groups:normal control group were without any treatment (NC); diabetes model control group(DM); traditional Chinese medicine prevention and cure group(ZY) and the insulin control group(XY). Except the normal control group, the other three groups were treated with intraperitoneal infection 55mg/kg of streptozocin (STZ). The rats in traditional Chinese medicine prevention and cure group were treated with Bushen Tongqiao Recipe lml pre-treating 1 week before the day STZ was injected for five weeks continuously. The insulin control group was cured by insulin 2u since the day the model was established while the rats in other two groups under the amount of saline filling. Observe the spirit form each rat related symptoms etc. At the same time of every week, the weight and fasting blood-glucose of the rats were detected.40 rats were killed four weeks later, fixative was injected intravascularly into the brain and tissues were picked up to make into continual paraffin was sections. The Bax and the Bcl-2 expression level was elaluated by immuno-histochemistry SABC. Totaling the number of the positive expressions as their positive cell's density with ocular reseau of optical microscope.Results (1) Compared with NC group, the rat's reaction and action of DM group were stupid, the weight was lower (P<0.01), the fasting blood-glucose of the rat was higher than that of the DM group (P<0.01), the positive cell's density of the expressions of Bax increased in Hypothalamus Anterior(A) and Lareral septal nucleus (L)areas of the DM group (P<0.05) while the positive cell's density of the expressions of Bcl-2 decreased (P<0.05). (2)Compared with the DM group, the ZY group's reaction and action were smart and the weight was higher(P<0.01)the fasting blood-glucose was lower of rats in the DM group(P<0.01), the positive cell's density of the expressions of Bax decreased in Hypothalamus A and L areas of ZY group (P<0.01)while the positive cell's density of the expressions of Bcl-2 increased(P<0.01). (3)Compared with the XY group, the positive cell's density of the expressions of Bax decreased in Hypothalamus A and L areas of ZY group(P<0.05) while the positive cell's density of the expressions of Bcl-2 increased<0.05).Conclusions It was suggested that modified Bushen Tongqiao Recipe was effective in preventing and treating the degeneration of the hypothalamus neuron of IT diabetic rats induced by STZ.