| Objective:To research the effects of rhizoma drynariae extract,icariin and pyritum on proliferation of rabbit stromal cell and study the mechanism of promoting the concrescence of fracture of traditional Chinese medicine. Method:Derived from bone marrow of trochanteric region of New Zealand rabbit and then cultured it after separated it with lymphocyte separation liquid,studied P2 generation and divided it into 4 groups(standard medium group,rhizoma drynariae extract group,icariin group,pyritum group) and cultured it separately, measuring its growth curve with MTT method and testing its mitosis index,studying the form of the cell with hematoxylin-eosin pigmentation. Then analyze the result with statistic method. Result:According to the statistic analyzation, there are quite remarkable difference between the rhizoma drynariae extract group,the icariin group and the standard medium, while there are no conspicuous difference between the pyritum group and the standard medium. Conclusion:The extracted liquid of pyritum group and the icariin group can inhabit the proliferation of the rabbit stromal cell while those groups can promote the proliferation of the rabbit stromal cell. No conspicuous evidence was founded that the pyritum medium has effect on the proliferation of the rabbit stromal cell. |
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