Constituents And Quality Standard Of Eucommia Ulmoides Processed With Salt-water

Eucommia ulmoides Oliver belongs to Zelkova, Eucommia Family, which is endemic to China, also known as silk with skin, is one kind of macrophanerophytes, with its skin and leaves as the main medicinal part. Eucommia ulmoides Oliver bark was regarded as rare medicinal herbs since ancient times, has the efficacy of gluten, strong bone, preventing miscarriage and blood pressure and other effects.Eucommia ulmoides processed with salt water is a Preparata procuct, which was included in the Pharmacopoeia of the P.R.C. The chemical constituents of Eucommia ulmoides were usually coveraged, but the salt products were relatively few. To clarify its active ingredients and medicinal efficiency, the application of modern chromatographic, spectroscopic techniques were used to study its chemical composition.8 compositons were isolated including bisepoxylignans 2 compounds:(+)pinoresinol di-O-β-D-glucopyranoside, DZ-1 and (+)-syringaresinol, DZ-5. Iridoid glycoside 2 compounds:geniposidic acid, DZ-2 and geniposidic, DZ-3. Phenylpropanoids 1 compounds:chlorogenic acid, DZ-4. Other terpenoids:Betulin, DZ-6 andβ-sitosterol, DZ-7 andβ-daucosterol, DZ-8.Pinoresinol diglucoside (PDG) is the main hypotensive constituents of Eucommia ulmoides. The HPLC determination contained in Pharmacopoeia of the P.R.C (Edition 2005, Volume I), and in Pharmacopoeia of the P.R.C. (Edition 2005, Volume I) the salt procuct was added as the quality control system of Eucommia. However, the experiment found that by changing the chromatographic conditions, the word "Pharmacopoeia" method in the PDG targets a peak separation of content accounted for 7.54%of impurities. In view of this system was found to re-establish the salt content in Eucommia PDG for HPLC determination and added the TLC scanning. This new HPLC method for determination took a longer analysis time, as a result, the ultra-high performance liquid chromatography on Eucommia ulmoides herbs and salt content of products in the PDG was used. In the similar chromatographic conditions, the new method reaches the aim of time saving, solvent saving and acquires the high efficiency.