Study On Injection Of Effective T Lymphocytes Inhibited Ectopic Endometriosis Development

Endometriosis is considered one of the most frequent diseases in gynecology, affecting10 -15% of women in their reproductive age. The main clinical features are infertility, severe dysmenorrhea, dyspareunia, chronic pelvic pain and abnormal menstruation. It is apparent that the disease rigorously interferes with the patients'quality of work and life. However, an in-depth understanding of the cellular and molecular mechanisms involved in endometriosis is being uncovered, and the inadequate immune responses in the pelvic microenvironment are significantly involved in the pathogenesis of endometriosis. Immune tolerance plays a central role in the implantation and development of exfoliated endometrial cells. In order to use the power of immune surveillance mechanism, prevent ectopic endometrial cells to evade the immune system, the bone marrow-derived dendritic cells (BMDCs) were isolated from inbred line rats, to present the antigen of rat ectopic endometrial cells for introducing proliferation of T lymphocyte. After generation of experimental endometriosis by ectopic endometrial implantation in the peritoneal cavity, the effective T cells were inject into the abdominal cavity of rats (n=8). Result showed that there was a significant difference about the atrophy in the ectopic lesion of 3 rats in experimental group with the normal models (p<0.05). And we found one rat with severe pelvic Inflammatory reaction. These results suggested that Injection effective T lymphocytes into rats with endometriosis could evidently inhibited the ectopic lesion development. Cytotoxic T lymphocyte (CTL) could be induced to clear the ectopic endometrial cells by the immune response. Changing the pelvic microenvironment with increase the power of immune surveillance may be an effective therapeutic method, which is an important base of autovaccine research to endometriosis.