The Function Of Chip In The Process Of Tumorigenesis In The Esophagus

The C-terminus of Hsp70 interacting protein (CHIP) is being considered to be a cellular protein quality control E3 ubiquitin ligase because of its ability to degrade misfolded proteins in association with heat shock chaperones, serve as the ATP-dependent selective protein degradation pathway-ubiquitin proteasome pathway. In malignant cells, the oncogenes and anti-oncogenes are expressed abnormally, which induce the abnormality of the ubiquitin proteasome pathway necessarily, then the CHIP is expressed abnormally. Screening the expression profile of CHIP gene in normal tissues and cancer tissues will elucidate the relationship between the abnormality of ubiquitin proteasome pathway and the development of cancer; also it will be helpful for cancer diagnosis.We detected the expression profile of CHIP in normal tissues and cancer tissues from esophagus and stomach at two levels, one is the level of transcription, and another is the level of translation. At the level of transcription, we use the agarose gel electrophoresis and Real-time Qualititative RCR to analysis the amount of the mRNA of CHIP in different tissues; at the tanslation level, we use the technology of immunohistochemistry to detect the amount of CHIP in different tissues.We found that CHIP protein is overexpressed in transcription level and translation level in esophagus cancer. It demonstrated that CHIP expression in esophagus cancer might be regulated by transcriptional and translational mechanism. Our data implicate that CHIP may serve as a valuable molecular marker for esophagus cancer.