Inactivation Of Enterovirus With Combined Peracetic Acid/uv Treatments

PV is the representation of enterovirus and have quite high resistance to disinfectant. The biological property of coliphage f2 is similar with HAV&PPV, and its resistance to disinfectant is similar with pathogenic enterovirus. This study try to evaluate the disinfection efficacy by using disinfectant to sterilize these two virus. PAA has stong killing effect on bacterium and fungi. UV has strong inactivation effect on bacterium and virus, and disinfected water is safe. This study used live attenuated virus vaccines and coliphage f2 as indicator of virus in disinfection of drinking water, prepared simulated water sample, sterilized the virus by PAA combined with UV, then compared the disinfection effect with sodium hypochlorite's and evaluate it, provided reference data for the disinfection of virus in drinking water.Part I Study on the Inactivation Effect of PAA-UV on Poliovirus Type IObjective: Studied on the inactivation effect of PAA combined with UV on poliovirus type I , then compared it with sodium hypochlorite and evaluated its effect. Method: Proliferated PV-I, got the supernatant and prepared simulated water sample. Tested the infectivity titer of PV-I by the end point dilution method. Chose effective concentration of neutralizer through neutralizer identified experiment. Used suspension quantitative inactivated test to disinfection in water sample. Added neutralizer into the solution to finish the experiment. Tested its concentration, and calculated the value of reduction.Result: The results showed that it could achieve 1.75±0.08 log PV-I reductions when PAA at dosage attaining 3mg/L, ultraviolet irradiation attaining 10 mws/cm2 with a 10min contact time. The combination of PAA and UV treatments caused more than 4 log PV-I reductions when PAA at dosage attaining 3mg/L, ultraviolet irradiation attaining 19 mws/cm2 last about 10 minutes. The inactivating efficacy of sodium hypochlorite was not strong, while 0.31±0.19 log reductions of PV-I at a 3mg/L available chlorine dose with a 10min contact time.Conclusion: The combination of PAA and UV treatments have stronger efficacy on PV-I than sodium hypochlorite , and the inactivating efficacy increased with increasing the dosage of PAA and UV.Part II Study on the Inactivation Effect of PAA-UV on Coliphage F2Objective: Studied on the inactivation effect of PAA combined with UV on coliphage F2, then compared it with sodium hypochlorite and evaluated its effect.Method: Proliferated coliphage f2, got the supernatant and prepared simulated water sample. Tested and calculated the concentration by double agar cultured method. Chose effective concentration of neutralizer through neutralizer identified experiment. Used suspension quantitative inactivated test to disinfection in water sample. Added neutralizer into the solution to finish the experiment. Tested its concentration, and calculated the value of reduction.Result: The results showed that it could achieve 0.54±0.13 log coliphage f2 reductions When PAA at dosage attaining 3mg/L, ultraviolet irradiation attaining 10 mws/cm2 last about 10 minutes. The log reductions of coliphage f2 can achieve 1.62±0.84 when PAA at dosage attaining 15mg/L, ultraviolet irradiation attaining 27.9 mws/cm2, and the inactivated the log reductions of coliphage f2 can achieve more than 4 when PAA at dosage attaining 100mg/L, ultravioletirradiation attaining 27.9mws/cm2. The sodium hypochlorite only caused 0.25±0.04 log reductions of coliphage f2 at a 3mg/L available chlorine dose with a 10min contact time.Conclusion: The combination of PAA and UV treatments have stronger efficacy on coliphage f2 than sodium hypochlorite , and the inactivating efficacy increased with increasing the dosage of PAA and UV.