The Corelation Between Smad4 And Intrahepatic Cholangiocarcinoma Progression

Objective To investigate the clinical pathology of ICC, for the purpose of providing preliminary clinical evidence for further study of ICC. Method 97 cases of ICC of clinical and pathological information were retrospectively analyzed in Wuhan Tongji hospital from April 1985 to December 2008 . Results 1. 43.98% (42/97) of patients with hepatitis B surface antigen positive in which positive rate of males (51.61%) was significantly higher than female group (28.57%). The difference was statistically significant (P <0.05). 2. The primary tumor in the left lobe of ICC with liver metastasis 67.27% (37/55) significantly higher than the primary tumor in the right lobe 40.48% (17/42). The difference was statistically significant (P <0.05); 3. It was significant (P <0.05) that moderate-poorly differentiated tumor (66.67% ,34/51) tend to metastasis than high differentiated tumor (43.48% ,20/46). 4. Recurrence rate was 27.85% (22/79) within one year,and relapse group with blood transfusion rate (63.64%,14/22) was significantly higher than non-recurrent group (31.58%,18/57). Univariate analysis showed that blood transfusion is a risk factor for recurrence. Conclusion 1. In the patients suffering chronic hepatitis B or with positive serologic HBV, male patients has greater risk to develop into ICC. 2. Metastasis of ICC is associated with primary tumor location and differentiation. 3. Blood transfusion in operation was one of risk factors for tumor relapse. Objective To preliminary investigate the corelation between Smad4 protein and ICC. Methods Expression of Smad4 was detected in ICC with 49 cases of by streptavi Biotin-peroxidase complex method (SABC). The expression of Smad4 was compared with the clinical stages and pathological grades of the patients. Results 44.90% (22/49) were negative for Smad4 immunoreactivity in the tumor cells, while the adjacent, normal hepatic cells remained positive. In groups with moderately-poorly differentiated, high TNM stage, lymph node metastasis and intrahepatic metastasis showed significantly lower expression. No significant correlation was found between smad4 protein downexpression, gender, age, pathological type, tumor size and recurrence. Conclusion The abnormal expression of Smad4 in ICC suggested TGF-β/Smad4 signaling pathway may play an important role in the process of tumorigenesis and progress; Significant correlation was found between Smad4 expression and ICC differentiation, pTNM, intrahepatic metastasis and lymph node metastasis.