The Effect Of Musk-aconite-earthworm-pill On Sod,inos Of Synovium In Rabbits With Koa

Objectives The purpose of the experiment is to observe the influence of Musk-Aconite-Earthworm Pill on SOD and iNOS in synovial membrane in rabbit knee osteoarthritis, and discuss the possible mechanism of action for curative effect of Musk-Aconite-Earthworm Pill on osteoarthritis, and therefore supply experimental basis to its clinical application.Methods 1.Divide the rabbits into 4 groups and establish model: number the 40 healthy male Japanese white rabbits according to their weight and divide them at random into 4 equal groups: Group A: normal comparison group; Group B: model group; Group C: Musk-Aconite-Earthworm Pill group; Group D: osteoproliferation resistant tablet group. And then establish model of experimental knee osteoarthritis with intra-articular injection of papain(1.6%) physiological saline solution .2.Provide medicine: provide in gastric perfusion way 6 times Musk-Aconite-Earthworm Pill solved in 10ml physiological saline solution of the quantity per day and per kg weight of for an adult to Group C; and provide osteoproliferation resistant tablet in the above method to Group D once per day. And provide Group A and Group B with equal quantity of physiological saline solution in gastric perfusion way. Continue this for 4 weeks.3.Observe the expression of SOD and iNOS by immunohistochemistry.Results Expression of SODand iNOS: The positive SOD,iNOS located in cytoplasm, brownish or brownish granule. Mainly distributed in synovial lining and synovial below. Compared with group A,expression rates of SOD in group B increased (P<0.01),compared with group B, SOD in group C and D increased(P<0.01),The study showed no statistically significant in group C with group D (P>0.05);iNOS is no expression in group A,Compared with group B, iNOS in group C and D decreased (P<0.01), The study showed no statistically significant in group C with group D (P>0.05).Conclusion Musk-Aconite-Earthworm pill can up-regulate the expression of SOD and down-regulate the expression of iNOS.