Quality Reasearch And Quality Standard Establishment In 2010th Chinese Pharmacopoeia Of Chaihu

Object:The main purpose of this paper is to revise the quality standard in 2005th Chinese Pharmacopoeia of chaihu and establish its 2010th quality standard. Deep research should be done to control chaihu's quality and make sure of its safer and more effective useage.Methods:Make use of standard methods in the appendices of 2005th Chinese Pharmacopoeia to determinate traditional quality guidelines of crude drug, herbal pieces and stir-fried with vinegar of chaihu (each with at least 10 samples), such as water content, total ash content, acid undissolved ash content, alcohol dissolved extractives content; HPLC is applied to determine the content of SSa, SSc and SSd of the samples. TLC, HPLC and NIR are used to find out the difference of Bupleunum chinese DC. and B. Scorzonerifolium Willd. Determine the content of SSa, SSc and SSd of the sample, using the relative correction factor of SSa to SSc (a method of analysis multi-components with single marker) and SSd, and compare with the results determined by external standard method. HSCCC was used to abstract the active compound of chaihu.Results:The content of water, total ash, acid undissolved ash, alcohol dissolved extractives, SSa, SSc and SSd of the samples were determined, and their standard content was limited. According to the atlas got by TLC, HPLC and NIR, distinct difference was found out between Bupleunum chinese DC. and B. Scorzonerifolium Willd. No significant difference was found about the content of SSa, SSc and SSd of the sample determined by the method of analysis multi-components with single marker and the external standard method. SSc was successfully extracted from the Radix Bupleuri. by using HSCCC.Conclusion:1. Establish the 2010th Chinese Pharmacopoeia of chaihu;2. Establish the HPLC determination method of SSa, SSc and SSd in Radix Bupleuri;3. Distinct difference was found out between Bupleunum chinese DC. and B. Scorzonerifolium Willd. By using TLC, HPLC and NIR.4. Establish the method of analysis multi-components with single marker to determine the content of SSa, SSc and SSd of Radix Bupleuri.5. Establish the method of extracting SSa from Radix Bupleuri. by using HSCCC.