Telomere Shortening And Dna Damage In Colorectal Cancer

Colorectal cancer (CRC) is derived from intestinal epithelium, and is one of the most common gastrointestinal malignancy. In China, the incidence rate was gradually rising with improved living standards, diet changes. Recent studies have found that change in telomere length is an important indicator of cancer, and monitoring change in telomere length is an important means of cancer research. In this study, we use quantitative fluorescence in situ hybridization (Q-FISH) to determine telomere length of colorectal cancer cells, and use enzyme-linked immunosorbent assay (ELISA) and chemiluminescence to determine concentration of protein associated with DNA damage and telomere shorting (Stathmin, EF-1α, n-acetyl-glucosaminidase, Chitobiosidae)in serum of patients of colorectal cancer.Through comparing correlation of the results and the clinical parameters in colorectal cancer, we understand the role of telomere shortening and DNA damage in the development of colorectal cancer, the relationship with the prognosis of colorectal cancer, so that we can search for better tumor markers of clinical prognosis. We find that statistical significance of telomere length of colorectal cancer and Dukes stage is positive, and DNA damage and telomere shortening associated protein in the serum of colorectal patients is statistically related with colorectal liver metastases and CA199 expression, so it is further our understanding of telomere shortening and DNA damage in the process of colorectal cancer, and provide the experimental evidence to some extent in predicting the prognosis of colorectal cancer.