The Association Between Relative Telomere Length Of Peripheral Blood Cell And Hypertension In Coal Workers

Objectives:The purpose of this study is to explore the relationship between the telomere length of peripheral blood leukocytes and essential hypertension about coal workers.The study further provides the prevention of hypertension for coal workers.Methods:1.Using QIAGEN TIA Namp Blood Mini Kit(51104)kit to extract the tested subjects' DNA from their leukocytes.2.Using Real-time quantitative PCR to test the length of telomere and calculating the ratio of the ct value of telomere to the ct value of single copy gene.This ration means the relative length of telomere.3.The correlation between telomere length and hypertension was analyzed by SPSS19.0.Results:1.The study was a paired design experiment,with 230 cases of hypertension,and 230 cases of healthy people.The mean systolic blood pressure was 145.73±16.99 mm HG,the mean diastolic blood pressure was 91.87±14.37 mm HG,the mean systolic pressure was 120.07±10.23 mm HG in the control group,and the mean diastolic pressure was76.24± 8.71 mm HG,and there was a significant difference between the two groups.2.There was no significant difference between hypertension group and control group in age and height;while there were significant difference in TC,GGT,GLU,HDL,LDL,TG,TP,BMI,between hypertension group were higher than the control group.3.3.The range of telomere length of peripheral blood leukocytes is 0.2149-1.9531 in hypertension group.While in control group the range of telomere length of peripheral blood leukocytes was 0.4562-2.1673.Peripheral blood leukocyte telomere length and age have negative correlation.(P<0.05).The average telomere length of female hypertensive patients was 1.2613 ± 0.4112,and the average telomere length of male patients with hypertension was 0.9981 ± 0.3965.By T test,there was a significant difference in telomere length between male and female hypertension patients(P<0.05).Conclusion:1.Cases of hypertension group in the total cholesterol(TC),glutamyl transpeptidase(GGT),glutamate(GLU),high density lipoprotein(HDL),low density lipoprotein(LDL),triglyceride(TG),total protein(TP),BMI index was higher than the control group.The value of two groups have statistical difference.2.Peripheral blood leukocyte telomere length and age have negative correlation.The average telomere length of male hypertension patients is lower than that of female hypertension patients.3.The telomere length of hypertension population is different from that of normal population,and the relative telomere length of hypertension group is shorter than that of normal population.