Clinical Significance And Expression Of Hsp27/60 And P53 In Gastric Cancer

ObjectiveTo study the expression of HSP27/60 and p53, explore the relationship between HSP27/60, p53 expression and clinicopathological features in human gastric cancer; The aim of this study was to investigate the impact of HSP27/60 and p53 on the development of gastric cancer, and its role for the judgement of the prognosis of gastric cancer.MethodThe paraffin-embedded tissues of 48 surgically resected gastric cancer verified by pathology and 14 adjacent normal tissues were obtained from the Department of Pathology of Liaocheng People's Hospital. All patients were not given preoperative chemotherapy, radiotherapy or biotherapy. Using SABC immunohistochemical staining to examine the expression of HSP27, HSP60 and p53 in gastric cancer tissues and in adjacent normal gastric tissues. The expressions of them were statistically analyzed in relation to various clinicopathological characteristics, tumor proliferative capacity.Results1. In human gastric cancer tissues, the HSP27 expression, the HSP60 expression and the p53 protein expression were positive in 31 of 48 cases (64.6%),33 of 48 cases (81.3%) and 33 of 48 cases (68.9%) respectively. The HSP27/60 expression were positive in 1 of 14 cases (7.1%), and p53 was negative in human normal gastric tissues.2. The expression of HSP27 was related to the depth of tumor invasion lymph node metastasis and TNM stage (P<0.05), while with no relation to the patients'sex, age, tumor size, location, differentiation grade, distance metastasis and tumors'proliferative capacity (P>0.05).The expression of HSP60 was related to differentiation grade and tumors' proliferative capacity (P<0.05), while with no relation to the patients'sex, age, tumor size, location, the depth of tumor invasion, lymph node metastasis, distance metastasis and TNM stage (P>0.05).The expression of p53 protein was related to tumor size, location, the depth of tumor invasion, lymph node metastasis and tumors'proliferative capacity (P<0.05), while with no relation to the patients'sex, age, differentiation grade, distance metastasis and TNM stage (P>0.05).3. Statistical analysis suggested a significant correlation between HSP27 and HSP60(r=0.314 p=0.032). p53 was not correlated with HSP27 or HSP60 (HSP60 p53 r=0.252 P=0.084, p53 HSP 27 r=0.159 P=0.282)Conclusion1. HSP27/60 and p53 protein positive expression rate was higher in gastric cancer than normal gastric tissues.2. The expression of HSP27 was related to the depth of tumor invasion, lymph node metastasis and TNM stage (P<0.05).The high expression of HSP27 indicated high malignant gastric cancer and a poor prognosis for a gastric cancer patient.3. The expression of p53 protein was related to tumor size, location,the depth of tumor invasion, lymph node metastasis and tumors'proliferative capacity (P<0.05).They indicated high malignant gastric cancer and a poor prognosis for a gastric cancer patient.The expression of p53 correlated well with gastric location.This result may show that the cancers of cardia have distinct pathogenesis and molecular characteristics from that observed in cancers of antrum4. The expression of HSP60 was related to differentiation grade and tumors' proliferative capacity (P<0.05), there was no available data concerning the clinical significance of HSP60 expression in relation to clinicopathological parameters in gastric cancer.5. The expression of HSP27 and HSP60 in gastric cancer is positive correlation. This indicated that they could prompt mutally in gastric carcinoma progression. The expression of p53 protein in gastric cancer didn't correlate to that of HSP27 or HSP60. Combined detection of HSP27/60 and p53 protein in gastric carcinaoma maybe helpful for evaluation of patient's condition and prediction of prognosis...