CXCR1Related With Clinical Pathological Features Of Gastric Cancer And Its Related Protein And The Functional Verification Of Distinct Protein HSP27

Objective:CXCR1and its relevant relationship between protein and clinical pathological features of gastric cancer and HSP27affect migration and apoptosis of gastric cancer.Methods:Using immunohistochemical method to detect CXCR1, HSP27, HNRNPK and NM23, KERATIN8, sorcin in gastric cancer and the expression of carcinoma, expressed in statistical software spssl7.0statistical analysis of each factor and each factor and the sex, age, grade and whether there is the relationship between lymph node metastasis, further analysis CXCR1factors associated with each. Build four short hairpin eukaryotic expression vector for HSP27, import it into MKN45anthropogenic sex immortalized gastric cancer cells, using western blot and rea-ltime-polymerase chain reaction (PCR) to screen out of HSP27gene inhibits the strongest carrier si-HSP27-MKN45. Cells can be divided into three groups, the blank group (CON), the control group (NC) has nothing to do these NC for transfection fluorescent sequence of siRNA and low expression of HSP27cell line (si-HSP27-MKN45). With Nick experiment, transwell experiment and flow cytometry to detect cell migration, invasion, and apoptosis. Using western blot method to detect HSP27expression of threegroups of ceelsResults:The six kinds of protein positive expression in gastric cancer and adjacent cell membrane or cytoplasm, but different strength weak level., using rank analysis method of nonparametric test, in addition to every index by cancer and cancer of HNRNPK expression differences are significant (P<0.05). The five kinds of proteins expressed in cancer is better than tissue adjacent to carcinoma, HNRNPK beside carcinoma and the expression of no statistically significant difference (P>0.05) with chi-square test method, the various indicators of expression level in terms of gender and age are not statistically significant (P>0.05). SORCIN, HSP27, HNRNPK, CXCR1expressed in patients with lymph node metastasis than without lymph node metastasis in patients with difference was statistically significant (P<0.05), Keratin8and NM23expressed in patients with lymph node metastasis is lower than patients with no lymph node metastasis, the difference was statistically significant (P<0.05). Spearman hase.Close sex analysis SORCIN, HSP27, HNRNP, CXCR1and gastric cancer classification into positive correlation (P<0.05), NM23, KERATIN8and classification of gastric cancer unrelated (P>0.05). CXCR1and HSP27, SORCIN, HNRNPK, NM23, KERATIN8are positively related (P>0.05).Compared with CON group and NC si-HSP27-the amount of the expression of HSP27MKN45significantly decreased (P<0.05) in cell migration, invasion decreased (P<0.05) cell apoptosis reduced. And si-CXCR1expression of HSP27-MKN45lower (P<0.05).Conclusion:1. The six factors except HNRNPK external expression of stronger than carcinoma, and had nothing to do with the age and gender. Sorcin, hsp27, hnRNP, CXCR1expression is higher than in patients with lymph node metastasis in patients with no lymph node metastasis, Keratin8and Nm23expressed in patients with lymph node metastasis is lower than patients with no lymph node metastasis. SORCIN, HSP27, HNRNP, CXCR1and gastric cancer classification into positive correlation and nm23, Keratin8and classification of gastric cancer irrelevant. CXCR1and HSP27, SORCIN, HNRNPK, NM23, KERATIN8are positively related (P>0.05).2. HSP27can enhance the invasion of the gastric cancer cell migration and apoptosis resistance.