The Study Of High-risk Hpv Infection And Hterc Gene Expression In Cervical Epithelial Cells

ObjectiveCervical Cancer is a malignancy which has been a serious threat to the health of women. It stands the first place in reproductive tract malignancies. In recent years, the incidence of Cervical Cancer seems to increase rapidly and it tends to happen to younger generation. These make the situation of prevention, diagnosis and treatment more serious. Cervical Cancer is characterized by precancerous lesions, that is, cervical intraepithelial neoplasia (CIN). Therefore, it is very important to detect the early cervical intraepithelial neoplasia patients and give them early treatment. Recently, the research against Cervical Cancer taken directly by the U.S. National Institution of Health suggests that the process of atypical cervical cells from cervical dysplasia to cancer is nearly all accompanied by the long arm of chromosome 3 amplification. The most important gene involving in may be Human telomerase gene (hTERC gene, located 3q26.3). The gene amplification can prevent apoptosis and lead to tumor production.We use FISH technique to test the cervical exfoliated cells hTERC gene expression and investigate the expression circumstances of high-risk type of HPV infection and gene expression in different hierarchy cervical lesion cells.MethodsFluorescence in situ hybridization (FISH) were used to detect the expression of hTERC gene in 140 cases of exfoliated cells of cervix (20 cases of normal cervix, CINI 29 cases, CIN II 31 cases, CINIII 27 cases and 33 cases of invasive cervical cancer). And detect the HPV-DNA of cervix tissue by using flow-through hybridization and gene chip technology in the Forth Affillated Hospital of China Medical University from July.2009 to Feb.2010. ResultsThe infection rates of high-risk type HPV in CINI, CINⅡ, CINIII and cervical cancer are 10.30%,48.40%,88.90% and 91.00%. Cervcal lesion and cervical squamous epithelium with high-risk HPV infection show hTERC gene amplification. The positive expression rates of hTERC gene in normal cervix,CINI, CINⅡ, CINⅢand cervical cancer are 10.00%,20.70%,58.10%,85.20%,97.00%. The high-risk HPV infection and the positive rate of hTERC gene raise with the gradual upward. High-risk HPV infection and hTERC gene expression in cervical cancer group and CIN groups and normal control group are significantly different. (P<0.05). The positive rate of HPV in hTERC (+) is significantly higher than in hTERC (-). Expreesion of hTERC gene is closely related to infection of high-risk HPV(rs=0.794, P<0.05).ConclusionshTERC gene amplification in cervical lesions and high-risk type HPV infection was positively correlated, hTERC gene over expression in cervical cancer, may play an important role in development.It is possible to diagnose cervix in an early stage and predict the process of cervcal lesion by using FISH technology to detect hTERC gene expression in cervical exfoliated cells.