| Objective:To investigate the efficacy of insulin on ischemic random dorsalflap in the SD rats,and discuss the protection of the ischemic-reperfusion injury,theenhancement of viability and the correlation between them on the ischemic randomflap of rats.Methods:A dorsal,caudally based random flap measuring 6.0cm×1.5cm wascreated,which were divided into 2 groups:treated group(Group A n=16) with singledoseinsulin 0.1U(1ml) injected under the flap afer operation;control group(Group Bn=16) with saline. Before injection and 1h,2h,3h after injection,levels of bloodglucose were measured,at 24th hour postoperatively,the amount of superoxidedismutase (SOD),glutathione(GSH) and malondialdehyde(MDA) in the flap tissuesamples were measured;the flap viability and microvascular expression weremeasured at 7 after operation.Results:There was not a significant decrease in blood glucose in treated groupcompared with control group(P＞0.05); at 24th hour postoperatively,there was a very significant decrease in MDA in treated group compared with control group,and asignificant increase in SOD ,GSH (P＜0.05); at 7 after operation,the viability intreated group was very significantly higher compared with the controlgroup(73.963±8.649)% vs(56.175±4.250)%,(P＜0.01).ConclusiConclusion:Topical use of insulin markedly improved the viability of ischemicrandom dorsal flap of SD rats,which may be the result of the reduction of ischemiareperfusioninjury. Insulin may improve the compaliance of the flap by theangiogenesis effect. |
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