| objective: this experiment is mainly examine the effect of he-nelaser irradiation to promote skin flap survival by studying the generalobservation flap survival area, neutrophil count, microvascular count in a longflap model which in the back of rats and the statistically compared with theexperiment without he-ne laser irradiation. this experiment also studying therelationship between the exposure range and effect of he-ne laser therapy bydividing the experimental group into3groups in which the first one wassubmitted to laser irradiation, using the punctual contact technique on the skinflap surface, the second one was submitted to laser irradiation surrounding theskin flap and the third one was submitted to laser irradiation both on the skinflap surface and around it. Methods: Experimentally controlled randomizedstudy.24healthy SD rats were used, all are female, weight300-350g, wererandomly divided into three experimental groups and one control group with6rats each. Each groups keep in different cages and are numbered. The Group B(control) underwent sham irradiation with He-Ne laser. The Group A1wassubmitted to laser irradiation, using the punctual contact technique on the skinflap surface. The Group A2was submitted to laser irradiation surrounding theskin flap, and the Group A3was submitted to laser irradiation both on the skinflap surface and around it. The rats were anesthetized by intraperitonealinjection of Ketamine (80mg/Kg), and then the long rectangle random skin flapwas performed measuring9x2cm which is located in the caudal. The experimental groups were submitted to He-Ne laser irradiation with2J/cm(2)energy density for15minutes at a time,24hours after the surgery and onceevery72hours.9days after the surgery, we take photography of the flap, andtake pathological specimens from it. These specimens were fixed for24hours,with ethanol dehydration step by step, paraffin embedding, the made into slices,control operating HE staining under strict conditions. Observation indexesincluding the flap survival area of the count, neutrophil count, capillaries. Aimage analysis software is used to calculate the skin flap survival area. Abinocular microscope is used to observe the sections with HE staining in orderto calculating the neutrophils.We calculat for then higher magnification viewand take the average as the record values.And we take the total of five highermagnification view of microvascular count as record values.The exclusioncriteria is: those blood vessels which has a bigger lumen and thicker smoothmuscle are considered as no-newborn blood capillary and won’t take in count.We use the statistical software SPSS13.0for statistical comparison. Results:1, gross observation: the general situation of the experimental group rats suchas activity, diet, etc, is better than that of control group.2.the flap survivalarea: the control group compared with the experimental group, P value is lessthan0.001, with statistical significance. It can imply that the he-ne laserirradiation therapy can effectively increase the flap survival rate. The flapsurvival area has no statistical difference in the Group A1which was submittedto laser irradiation, using the punctual contact technique on the skin flap surface compared with Group A2which was submitted to laser irradiation surroundingthe skin flap.(the P value is0.779, more than0.05). But the the flap survivalarea of the group A3which was submitted to laser irradiation both on the skinflap surface and around it is better than group A1and group A2with the P valueless than0.001, showing that irradiating both on the skin flap surface andaround it is better in adding the effect of skin flap survival rate of skin flap thanthat only illuminating skin flap area of skin flap surrounding area.3.neutrophils count: the control group compared with the experimental group, Pvalue is less than0.001, with statistical significance, implying that he-ne laserirradiation therapy can effectively reduce skin flap neutrophil infiltration aftersurgery.The flap survival area has no statistical difference in the Group A1which was submitted to laser irradiation, using the punctual contact techniqueon the skin flap surface compared with Group A2which was submitted to laserirradiation surrounding the skin flap.(the P value is0.943, more than0.05). Butthe the neutrophils count of the group A3which was submitted to laserirradiation both on the skin flap surface and around it is less than group A1andgroup A2with the P value less than0.001, showing that irradiating both on theskin flap surface and around it is better in adding the effect of educing skin flapneutrophil infiltration than that only illuminating skin flap area of skin flapsurrounding area.4.microvascular count: the control group compared withthe experimental group, P value is less than0.001, with statistical significance,implying that he-ne laser irradiation therapy can effectively increase the newborn blood capillary number skin flap after surgery.The microvascularcount has no statistical difference in the Group A1which was submitted to laserirradiation, using the punctual contact technique on the skin flap surfacecompared with Group A2which was submitted to laser irradiation surroundingthe skin flap.(the P value is0.679, more than0.05). But the the count of thegroup A3which was submitted to laser irradiation both on the skin flap surfaceand around it is less than group A1and group A2with the P value less than0.001, showing that irradiating both on the skin flap surface and around it isbetter in adding the effect of increasing the newborn blood capillary numberskin flap after surgery than that only illuminating skin flap area of skin flapsurrounding area. Conclusion:1.a certain dose of he-ne laser irradiation canimprove the skin flap survival rate, and irradiating both on the skin flap surfaceand around it is better in adding the effect than that only illuminating skin flaparea of skin flap surrounding area.2.a certain dose of he-ne laser irradiationcould reduce flap neutrophil infiltration, and irradiating both on the skin flapsurface and around it is slightly better in adding the effect than that onlyilluminating skin flap area of skin flap surrounding area, but there is nosignificant difference, we don’t exclude the possibility of experimental error.3.a certain dose of he-ne laser irradiation can promote the flap vascularregeneration, and irradiating both on the skin flap surface and around it is betterin adding the effect than that only illuminating skin flap area of skin flapsurrounding area. |
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