Investigation On Parasites In Goat And Sheep And Identification Of Cryptosporidium Isolates By Pcr-rflp Technique

To understand the prevalence of intestinal parasites in goats and sheep, from July 2006 to July 2007, a survey was conducted in four scale goat farms and five scale sheep farms in Henan province. A total of 1017 fecal speciemens from goats and 1265 from sheep were collected.The results showed that the average prevalence of parasites in goats was 94.69%(963/1017), and four species of parasites were found: Coccidia, Cryptosporidia, nematode and cestode. The corresponding infection rate were 93.90%, 2.75%, 45.13%, and 11.70%, respectively. Mixed infection of parasites was 54.72% in the positive samples. The ratio of mixed infection parasites included Coccidia, nematode and cestode was 8.52%. More than two species mixed infection shared the 45.90% ratio from the whole ifenction. In which, the infection of mixed Coccidia and nematode was the most serious, with a 44.96% ratio.Likewise, the average infection of sheep was 93.12% (1178/1265). A total of two species of protozoon (Coccidian and Cryptosporidium), two species of nematode (Trichuris ovis and Strongyloides stercoralis), and one species of cestode were checked out. Thereinto, the infection rate of Cryptosporidia, Coccidia, Trichuris, Strongyloides, and cestode were 3.10%, 88.94%, 10.83%, 59.11%, and 43.96%, respectively. The results also illustrated that the phenomonen of mixed infection in sheep was far more serious. For example, the mixed infection of Eimeria sp, tapeworm and Strongyloides stercoralis shared 60.10% of the total infections rate. Moreover, the results indicated that the infection rate of parasites was lower with the age increasing.Cryptosporidium can cause diarrhea of younger goats and sheep, and even to death. Due to some of the speices of ryptosporidium infected goats and sheep are zoonotic. Therefore, goats and sheep may be important resource of human cryptosporidiosis. In the present study, genotying was conducted by PCR-RFLP based on 18S rRNA gene. So we can understand the distribution of Cryptosporidium species or genotypes in goats and sheep in Henan province and further infer the significance of public health.28 goats Cryptosporidium isolates and 80 isolates from sheep were successfully amplified the 18S rRNA gene fragments by nested-PCR. The PCR products were respevtively digested by Sspâ… and Vspâ… restriction enzyme. According the to map of gel electrophoresis, three species Cryptosporidium cervine genotype (8 samples), C. bovis-like genotype (4 samples), and C. andersoni (16 samples) were identified in goats isolates. Likewise, in sheep isolates, three species cervine genotype (35/39), C. bovis (2/39), and C. andersoni (2/39) were also discovered. Therefore, the results showed that the Cryptosporidium cervine genotype are more often found in the goats and sheep, which indicates far more larger risk of zoonotic species.