Monitoring Of Digestive Tract Parasites Of Goats And Identification Of Coccidium And Cryptosporidium Species In Some Intensive Breeding Farms In Jiangsu

Parasitic disease is one of the main factors seriously endangering the stable development of sheep industry.It is widely distributed in the world,but has been neglected.In Jiangsu,mutton sheep is one of the most important industries in the breeding industry,and the production of mutton sheep is dominated by goats.Although parasitic diseases are widely recognized by everyone,their clinical manifestations are easy to hide under other diseases because they are mostly recessive infections,which seriously paralyze the awareness of breeding workers.The purpose of this study was to find out the infection types and infection situation of coccidium and cryptosporidium in goat digestive tract through the epidemiological investigation of the main parasites in goat digestive tract in intensive breeding farms in Jiangsu.From 2019 to 2020,a total of 446 fresh goat feces samples were collected from 8 intensive sheep breeding bases in 7 cities of Jiangsu Province(all of which were elevated beds).The parasitic infection of digestive tract of goats was detected by centrifugation precipitation method,mesh filtration method and saturated saline floating method.The results showed that the total infection rate of digestive tract parasites was 77.58%(346/446),and three kinds of digestive tract parasite eggs were detected,which were coccidioides,nematodes and tapeworms,among which coccidioides were the dominant species with an infection rate of 76.91%(343/446).The infection rate of different regions was analyzed.Gaoyou area had the highest infection rate of 96.43%(27/28).The second was Xuzhou,accounting for 83.33%(20/24).The infection rate of digestive tract parasites of goats in different seasons was the highest in summer.The infection intensity of diarrhea sheep feces samples and healthy sheep feces samples was compared.The infection intensity of diarrhea goats was significantly higher than that of healthy goats.In order to further understand the infection types and infection intensity of Eimeria in goats,an intensive goat farm in Haimen City,Jiangsu Province was selected for the investigation on the basis of the previous investigation.The fecal samples of goats in this farm were collected according to different months of age.Analysis of the data showed that the infection rate of juvenile goats was higher than that of lambs.Six species of Eimeria goats were identified after sporidized egg cysts with 2.5%potassium dichromate solution and microscopic observation for morphological identification.They were E.arloingi(78.9%),E.ninakohlyakimovae(72.2%),Ejolchijevi(68.9%),and E.christenseni(62.2%),E.alijevi(42.2%)and E.hirci(24.4%),with the dominant species being E.arloingi and E.ninakohlyakimovae;The analysis of the infection intensity of Eimeria in goats showed that the infection intensity was inversely proportional to the month age of the animals,and the younger the month age of the goats,the higher the average infection intensity was(P<0.05).Cryptosporidium pathogen was suspected to have been found during the early microscopic investigation.In order to understand the prevalence of Cryptosporidium in Jiangsu,the fecal genomic DNA was extracted from the fecal samples collected earlier,and the 18S rRNA gene primer of Cryptosporidium was synthesized.Nested PCR method was used to amplify the extracted fecal DNA samples.A total of 20 target genes of Cryptosporidium were amplified.The overall infection rate of cryptosporidium was 4.48%(20/446).The amplified gene fragments were recovered,purified and sequenced,and the species and genotypes of Cryptosporidium infection were identified by Blast comparative analysis of the target gene sequences.The goat Cryptosporidium species of the tested samples were C.ubiquitum and C.parvum.It was closely related to the cryptosporidium strains isolated from human host cases.The results showed that gastrointestinal parasites were prevalent in goats in intensive breeding farms in Jiangsu Province,and Eimeria was the dominant species.The species infected with goat cryptosporidiosis are C.ubiquitum and C.parvum,which may be a potential source of risk for human cryptosporidiosis.