| Fish soluble protein (FSP) is rich in protein, vitamin, mineral elements, amino acid and taste-active components. And it can promote the ingestion and growth of animal as well as enhancing the capability of anti-stress. The experiments are to study the effect of fish soluble protein as protein supplement to the growth performance and physiological and biochemical parameters of weaning piglets by the means of preliminary measurement of nutritive elements and the digestibility of them, choosing weaning piglets in 28±3d as subjects investigated. The experiments consist of 4 parts below:1. Analysis nutrient composition in fish soluble proteinThis experiment was to assay the nutrient element of organic compand, crude protein, crude fat, ash, amino acid and mineral elements of fish soluble protein (FSP). The result shows that the contents of crude protein, nitrogen free extract and ash of fish soluble protein were 64.08%, 11.60% and 19.99% respectively. The contents of each amino acid were lysine 3.29%, methionine 0.94%, glutamic acid 5.17%, alanine3.64%, glycine 3.45%, asparticacid 3.40%, threonine 1.65%, serine 1.43%, praline 2.25%, arginine 1.89%, leucine 3.17%, isoleucine 1.46%, phenylalanine 1.45%, histidine 1.92%, cysteine 0.42%, tryptophan 0.20% and autrine 2.50%. The total content of amino acid was 38.82% (autrine excluded), and the content of lysine was lower than that of imported fish meal among them. The contents of mineral elements were calcium 2.67%, phosphorus 1.12%, kalium 3.06%, sodium 2.36%, magnesium 0.45%, iron 850.50 mg/kg and zinc 21.36 mg/kg respectively. The ratio of calcium to phosphorus (Ca: P) was 2:1 approximately, but the content of calcium and phosphorus was lower than that of imported fish meal. The conclusion of this experiment is that the fish soluble protein is a premium animal protein source rich in protein, 18 anmino acid and mineral elements.2. Apprent digestibilty offish soluble protein including regular nutrient elements and amino acidThis experiment was choosing eight crossbred (Duroc×Landrace×Yorkshire) castrated boars with an original weight of 21.60±1.74kg to measure apparent digestibility of the regular nutrient elements of FSP and amino acid. The experiment was cross test by the means of collecting dung and cross-calculating. The result shows that the apparent digestibilty of organic matter, crude protein, nitrogen free extract, lysine, methionine, cysteine and total amino acid are 80.71%, 82.46%, 89.78%, 79.28%, 47.94%, 83.05% and 89.31% respectively. And the apparent digestible energy was 13.63MJ/kg. The conclusion of this experiment is that the fish soluble protein is a premium animal protein source and it could be assimilated easily by growing pigs.3. The effects of fish soluble protein on weaned pigs'growth performance and physiological and biochemical parameter in high quality formular dietThe experiment was conducted to evaluate the effects of fish soluble protein in high quality formular diet on weaned pigs' growth performance, physilogicla and biochemical characteristics. 120 crossbred (Duroc×Landrace×Yorkshire) weaned piglets (4 weeks old), with a weight of 7.57±0.84kg, were divided randomly into five groups with four replicates each which had six piglets. The primary stuff of diet was corn, extruded soybean, soybean meal, fish meal and whey powder. On the basis of performance protein mixed with feed, they were fed 0.00% (control group), 0.25%, 0.50%, 1.00% and 2.00% fish soluble protein instead of fish meal with equal protein. 5.00% fish meal was added in control group. The trial period was 30 days. The results showed that: (1) adding 1% fish soluble protein brought no negative effect on the growth of piglets, with feed consumption equivalent to that of the control group, and it was better than adding other amount. (2)The serum ALT and AST concentration of the group by adding 2% fish soluble protein was significantly higher than that of the control group, and the value increased with the adding amount of fish soluble protein. (3)Adding fish soluble protein brought no significant effect on the trypsin activity and chymotrypsin activity of piglet's duodenum. (4)When 1% and 2% fish soluble protein was added, the piglets' ileum villous height and the ratio between villous height and crypt depth (VH/CD) were higher than those of the control group (P＜0.05), and the ratio between jejunum villous height and crypt depth was higher than that of the control group (P＜0.05). Acorrding to the results of the trials, adding 1% fish soluble protein could instead of equal protein fish meal in high qulity diet. 4. The effect of fish solubles protein on weaned pigs' growth performance and physiological and biochemical parameter in plant protein fomular dietThe experiment was conducted to evaluate the effects of different fish soluble protein levels in plant protein diet on weaned pigs' growth performance, physiological and biochemical characteristics. 96 crossbred (Duroc×Landrace×Yorkshire) weaned piglets (4 weeks old), with a weight of 8.40±0.63kg, were divided randomly into four groups with four replicates each which had six piglets. The formular diet of control group consisted of corn, extruded soybean, soybean meal etc. On the basis of diet of control group, 0.40%, 0.80% and 1.20% fish soluble protein was respectively added in the plant protein feed with lower energy level than that of the 3rd trial. The trial period was 30 days. The results showed that: (1) The average day gain (ADG) of piglets adding 1.2% fish soluble protein was 0.45kg/d, 12.50% higher than that of the control group, and there was significant difference (P＜0.05). The average day feed intake (ADFI) of piglets adding 1.2% fish soluble protein was 0.75kg/d, 5.63% higher than that of the control group, but there was no significant difference (P＞0.05). (2)The serum HGH of piglets adding 1.2% fish soluble protein was higher than that of the control group significantly(P＜0.05); (3)The chymotrypsin activity of piglets fed 1.2% fish soluble protein was increased by 15.49%, but there was no significant difference (P＞0.05). (4)Their ileum villous height was 600.72nm, 20.89% more than that of the control group, which was 496.91nm. The results of the trial showed the growth performance and feed intake of the weaned piglets can be increased by adding 1.20% fish soluble protein in the plan protein feed; The fish soluble protein can accelerate and improve the mucous membrane of piglets. When the fish soluble protein was added in the plant protein feed, the trypsin activity and chymotrypsin activity of piglet's duodenum increased. |
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