Guizhou Different Habitats In Soil Microbial Structure Differences

Collected soils from different environments in Guizhou province and mensurated the water content and pH of them. The water content and pH of ZY01,ZY02,ZY03 were 21.2%, 19.9%, 17.7% and 6.5,6.5,6.0 respectively. Then (l)Cultivated and numerated the bacteria,fungi and actinomyces from soils with soil extraction media, beef extraction-peptone media, PDA media and Gause No.l synthetic media. There lived some bacteria, fungi and actinomyces in all the media described above. But the growth condition is different.The numbers of bacteria of g-1 of dried soils are 5.0x107, 3.0xl07, l.0xl07 for ZY01,ZY02,ZY03, by the beef extraction- peptone medium, respectively, and 9.0x 107, l.0xl07 for ZY02, ZY03, respectively. The numbers of fungi of g-1 of dried soils are 3.0x104, 1.5xl04, 2.5xl04 for ZY01,ZY02,ZY03, by PDA medium, respectively. The numbers of actinomyces of g-1 of dried soils are l.lxl06, 7.5xl05, 8.0xl05 for ZY01,ZY02,ZY03, by Gause No.l synthetic medium. (2)Ground the soils into powder after being dried or cold-vacuum dried. Then tried to extract DNA from these soils with three different methods, except of S02, all successfully extracted DNA, although the extraction efficiencies were different.The yield of crude DNA of g-1 of dried soils for ZY01,ZY02,ZY03 is from 2.5μg to 31μg. The purification degree of crude DNA extracted by method No.2 is the highest. (3)Purified the crude DNA extracted with the first method with the genomic DNA purification kit manufactured by Dingguo biotechnology limited company. Then amplified the sequence of 16SrDNA with PCR.