Cow House Space Microbial Count Medium Fast Design And Optimization

China is located in terms of dairy production consumption in the world, but in recent years,dairy safety problems occur frequently, where microbial contamination of raw milk production is an important source of pollution, not only for the quality of dairy cows and their health effects,more significant threat to the physical health of the consumer, and thus the production process of raw milk quality and safety has been widely concerned and researchers. To protect the health and safety of raw milk hygiene, improve the detection of microbial count rate, shorten the time microbial counts. The problem for the surrounding dairy Harbin microbial air sampler with air sampling seasons, and using matrix-assisted laser desorption time of flight mass spectrometry identification, identification of the results yielded the highest detection rate of pathogens Seasons belong to the Genus Bacillus: Bacillus Bacillus subtilis, Bacillus cereus; Enterococcus:Enterococcus faecalis, Enterococcus feces; Staphylococcus species: Staphylococcus xylose yield aureus; Acinetobacter spp: Loffi Acinetobacter baumannii; Corynebacterium: Corynebacterium xerosis. The results are consistent with those reported abroad.In this study, the nine pathogenic representative for the study, with reference to domestic and foreign media nutrients count in ordinary nutrient agar medium based on flour and add yeast extract, glucose, peptone and peptone replace with trypsin to prepare a substrate media. After optimization of the medium substrate ratio: Tryptone10g/L, glucose13g/L, yeast extract powder10g/L, beef extract 3g/L, Na Cl 5g/L.The substrate coating medium based on the counting method filter out nutrients promote the growth of common bacteria on behalf of: mannitol, sodium pyruvate, magnesium sulfate, biotin and tomato juice. Single-factor method to determine the appropriate concentration range for each growth-promoting substances, and then using response surface method of comparison between the growth promoting substance, each optimized growth-promoting substance concentration, and ultimately determine the ratio of the medium to produce a rapid count medium, thereby reducing space microbial count time and increase counting efficiency. Quick Count medium supplemented with growth-promoting substances results: mannitol, magnesium sulfate, sodium pyruvate, biotin and tomato juice. In this medium on behalf of the bacteria count by coating method, can be counted 10 h incubation, colony counting up to 1.28×109CFU/m L. Applications with air samplerswith the use of dairy actual sample taken back to count for 24 h, 48 h no difference with conventional count and count as NA medium half the time. Achieve a rapid count of purpose, at home and abroad to fill the blank space fast counting microorganisms, provide the basis for the realization of rapid detection of microbial quality and safety.