Effects Of Microwave Radiation On The Ecological Characteristics And Antioxidant Isoenzyme Of Sitobion Avenae (fabricius)

With the rapid development of both communication and power industry and the wide use of mobile phones, microwave ovens, computers and other electrical equipments, the electromagnetism in the environment strengthened greatly. This situation would inevitably put strong selective pressure on biological heredity and variation. So, it is of important practical value and theoretical significance to study how to make use of these radiations for pest control and the mechanism of pests'genetic evolution.Aphids as a kind of important agricultural pests, with small size, rapid reproduction, unique mode of reproduction, vulnerability to environmental stress and severe species differentiation and evolution, are considered as ideal material of insect ecology study.In this paper, cereal pest Sitobion avenae (Fabricius) was taken as experimental material and cultivated under different intensity of microwave radiation in order to explore the mechanism of microwave radiation's effect on aphids'growth, development, reproduction and other ecological characteristis. Meanwhile polyacrylamide gel electrophoresis (PAGE) method was used to investigate the impact of microwave radiation on aphids'system of antioxidant enzyme to discover toxicity mechanism of antioxidant enzymes under microwave radiation, providing theoretical basis for further study on the molecular mechanism of toxicity effect and microwaves'influences on ecological genetics and evolution of insects. Results are as follows:1. High-intensity microwave stress had a significant impact on the mean generation time, net reproductive rate, intrinsic rate of population growth and population growth potential of F2 generation of Sitobion avena, which indicated that the influence of microwave radiation on the the wheat aphid had inherited to next generation. Under different durations of microwave irradiation treatments, there were not significant differences, maybe the time frame selected was too short, and impacts of longer-term irradiation effects on the aphids need to be further studied.2. Microwave stress on wheat aphid's survival curves showed: in a certain exposure time, low-intensity microwave radiation increased the survival rate of Sitobion avenae, while, high-intensity microwave radiation increased the death of Sitobion avenae.3. Contemporary avenae induced by microwave stress, its offsprings'peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) isozymes of three kind of antioxidant enzymes changed in different degrees. SOD isozymes were obviously affected by microwave radiation, and three new bands of P3, P4, P5appeared, indicationg a certain intensity of microwave radiation activated the POD. Meanwhile, in terms of CAT and SOD isozymes, although there were no specific bands produced, the gray value variance analysis showed that the differences between treatment group and comparison group were significant, demonstrating that the microwave stress inhibited CAT and SOD activity.4. POD isoenzyme of wheat aphid was the most sensitive to microwave radiation, and played a major role in the stress response system, and CAT and SOD isozymes may function to some extent.This study is the first to explore the microwave effects on insects, and study results will provide theoretical basis for research on genetic and evolutionary ecology of insects and pest ecological regulation.