Phytosterol Succinic Acid Esterification Reaction And Recrystallization Of The Product Separation

Phytosterol is ond kind of important steroids. It has been reported that the hydrophilicity of phytosterol can be strengthened and the solubility in the water is also increased through modifying hydroxyl at C-3 position with succinic anhydride. Since phytosterol hemisuccinate are known to have a wide range of biological activility and physical properties, they are widely applied in the food, pharmaceutics and cosmetic industry. The hemisuccination process and kinetics ofβ-sitosterol and stigmasterol with succinic anhydride were studied, respectively. Synthesis of phytosterol hemisuccinate was also studied.The pure singleβ-sitosterol hemisuccinate and stigmasterol hemisuccinate were purified and separated from phytosterol hemisuccinate by crystallization method. The research results provide theorical basic and technological parameter for the industrialization of the process. The main research works were as follows:1. The hemisuccination process and kinetics ofβ-sitosterol and stigmasterol with succinic anhydrideThe reaction product ofβ-sitosterol and stigmasterol with succinic anhydride were characterized by elemental analysis, ¹H NMR and ¹³C NMR. It is confirmed thatβ-sitosterol hemisuccinate and stigmasterol hemisuccinate is the only reaction product, respectively.