Based On Raman Spectroscopy Of Dna Radiation Damage And Its Protection

DNA is important genetic armamentarium of life; it injured may be a direct result of the deaths life. The damage of DNA that caused by UV radiation can not be avoided by life. So the study on mechanism of UV damage to DNA, and the study on seeking a good Protection agent are very important. However, the existing biological ways that is used in research of DNA's damage mechanisms and the detection of a good Protection agent are not busy. In this study, Raman spectroscopy that is a physical technology platform is used in study, which is about UV damage to DNA and differences between different agents. We have made several attempts that be reported in this thesis.1. DNA in aqueous solution was ultraviolet radiated 25min and 45 min respectively; both conformation and configuration of DNA all are injured.2. The experimental results show that low concentration of vitamin C is in the protective role of DNA, high concentrations of VC damages DNA.3. Analyzing the Raman spectra of the DNA in aqueous solution that was ultraviolet radiated 15min, the experimental results show that mannitol can be a separate protection role in UV-induced DNA damage, and the higher its concentration is, the better its effect.4. Analyzing the Raman spectra of the DNA in aqueous solution that was ultraviolet radiated 15min, the experimental results show that DMSO can be a separate protection role in UV-induced DNA damage, and the higher it's concentration is, the better it's effect.5. In addition , this article also explored possible mechanisms of UV damage to DNA ,and the mechanisms of protection about that protection agents protect DNA.