A Novel Method For Studying On The Genetic Characteristics And Relationship In Different Fish And Other Animals

Currently, molecular genetic markers and DNA sequencing are mainly adopted for the analysis on genetic characteristics and the genetic relationship in different species. However, the methods mentioned above are either of taxing operation, or of low stability and repeatability, even in need of isotopes, while whole genome sequencing is undeveloped considering its high expenses and time-consuming. In this study, we employed a pair of degenerate primers of Sox (SRY-box) genes, via PCR (Polymerase Chain Reaction) and clone in the whole genome of different fish and other animals, we got a new molecular genetic marker which is simple and effective. It's also to be a novel method for studying on the genetics characteristics and relationship in different fishes and other animals.The Sox (SRY-box) gene family is a super gene family of HMG-box-containing genes whose predicted peptide sequence of the box shows >60% similarity to that of the SRY box. Each of the Sox genes encode a high mobility group HMG-box transcription factor, the amino acids motif is known to bind to specific target sequences in DNA. From Drosophila to mankind, the HMG-box of Sox genes were all presented highly conservation. We designed a pair of degenerate primers against the conserved region HMG-box in Sox genes, by PCR amplification, we obtained DNA fragments with definite sizes and numbers belonging to different Sox genes in many animals, including 19 natural fish species, 11 artificial diploid and polyploid hybrids, 4 amphibians, 2 crawlers, 4 Aves and 2 hexapods, from which we established molecular genetic markers for different animals. Furthermore, we sequenced 48 pieces of DNA fragments in 21 kinds of samples, especially those different samples which had similar DNA fragments appeared, in order to make a embedded analysis on their nucleic acid sequences. This novel method is simple, accurate and effective, applicable for study the genetic characteristics and genetic relationship of different animals.To be a new molecular genetic marker, the method can not only be adopted to identify different fish and other relative animals, but also be devoted to genetic variation analysis of animals, biological genetic breeding and animal resource investigation, and so on.