Not The Age Mice With Acute Hypoxia, Brain Tissue No Content Changes

Nitric oxide is a kind of gas which features in chemistry contain nonsymmetrical electron, simple structure, active property. In addition , it tend to diffuse . Moncada determined the endothelium-derived relaxing factor (EDRF) was the nitric oxide in 1978, since then, massive reports about nitric oxide and nitric oxide synthase have been provided each year. With the advance of the research about nitric oxide and nitric oxide synthase , more and more people start to focus on the content change situation of the nitric oxide under growth process and pathological state. Through exploring the content change of the nitric oxide in the acute hypoxia adaptation process in the brain organization of the different age segment mice, the paper provides some basic data about the function and the mechanism in mice growth process and pathological state.In this paper, we selects healthy mice of the species in Kunming, which are divided into three age segments: half-month mice; three-month mice and twelve-month mice (each 100) .We feeds the experimental animals a week in the laboratory guarding against the negative influence from the changing environment. The distribution of nitric oxide synthase and the change situation of nitric oxide content in mice acute hypoxia adaptation process are measured by the NADPH-d histochemical staining and the Griess method. The results are as follows:NADPH - diaphorase histochemistry was used to study the distribution of Nitric - oxide syntheses - positive neurons in the brain of mice. We can see that nitric oxide synthase masculine neuron widely distributes in the brain organization of mice and there shapes are rule. A single appliance was devised to serve as a model for mice under acute atmospheric narrow hypoxia situation in this paper. The new system could avoid the influence of high-concentration CO₂ or low pressure , was simple and cost2effective to make and convenient to use. The experiment of Griess method showed, nitric oxide unceasingly increases in the brain organization of mice along with the age growth. Mice may have the tolerance to the acute oxygen deficit, this kind of tolerance grows along with the age changing. Nitric oxide participates in brain organization to the acute oxygen deficit adjustment process, the nitric oxide content increases along with the increase of oxygen deficit time and the number.Nitric oxide is a mini-gas molecule with unstable property .The storage mechanism of nitric oxide is not sole. Once produced, t diffuses around and acts on neighbor by piercing cell membrane .Nitric oxide played a complicated and capital role during the anoxia of brain. During the anoxia of brain .the release of nitric oxide was controlled by nitric oxide synthase with the changes of nitric oxide ,and the activity of nitric oxide synthase was associated with the correlative factors of hypoxia .Nitric oxide played a protective and injury role during the anoxia of brain, which is determined by different times of anoxia of brain and the local content of nitric oxide. Proper dose of nitric oxide can protect brain tissues ; however ,In a great part of cerebral ischemia-hypoxia , inducible and neuron nitric oxide synthase should be used to inhibit the toxic effect of nitric oxide .The result confirms that the nitric oxide widely participates in mice's senile process and participates in the adjustment organism acute oxygen deficit to bear the stress. This result provided some basic data in animal organism function and the mechanism for further understood the nitric oxide. Simultaneously, it also provided some reference for the research of the nitric oxide and its preparation in clinical on application.