Microarray Analysis Of Arabidopsis T387 Mutant And Its Drought-resistant Mechanism Of A Preliminary Study

Water is very important for plant growth and development. It is not only as a medium for biochemical activities,but participating in cell metabolization directly. Loss of water will lead to the abnormity of metabolization. The terrestrial plant is not only exposed to water stress during drought stress and high salinity,low temperature also lead to the absence of water,roots can not provide enough water to leaves and reduce the turgor pressure in leaves. People pay much attention to the study of drought stress. It has significance in theoretical and practical aspects to study the physiological accommodation and explain the mechanism of tolerance to drought stress on molecular level.Recently cDNA microarray analysis has been developed for quantitative analysis of genome-scale gene expression and simultaneous analysis of expression profiles. This DNA chip-based technology arrays cDNA sequences on a glass slide at a density > 1000 genes/cm~2.These arrayed sequences are hybridized simultaneously to a two-color fluores-cently labeled cDNA probe pair prepared from RNA samples of diffenent cell or tissue types,allowing direct and large-scale copmarative analysis of gene expression.The Arabidopsis mutant t387 is resistant to nitric oxide. Gene chips were used to analyse the expression profiles of genes in Arabidopsis ecotype Col and t387 mutant under normal conditions. Compared to Col, we found 405 genes up-regulated and 269 genes down-regulated evidently in t387 mutant. We found that many stress related genes up-regulated in t387 mutant at normal conditions through searching the differencial genes in TAIR and NCBI and classifying with GO. We found t387 mutant is really resistant to drought stress and some up-regulated stress genes were validated at molecular level. t387 mutant has quick velocity of water loss and transpiration,big stoma aperature and low velocity of photosynthesis. Roots are very thick in t387 mutant,we presume that the resistant to drought is correlated with its developed roots.