Metal Ions And Their Complexes With Biomacromolecules Interaction Of Research And Analysis Applications

Organism is constituted by hundreds of millions of molecules with different molecular weight. Nucleic acids and proteins are the most important biomacromolecule. Nucleic acid is the carrier of genetic information. It is also the determination factor for species' continuation and evolution. Proteins are the carrier of many physiological functions, and are also the direct expresser of physiological characters. Protein play an important role in the metabolism of human body, and the most disease of human being is caused by the abnormal of protein. So the quantitative analysis of nucleic acid and protein are important in life science, biochemical medicament, food analysis and clinic analysis. It is very important for researchers to explore the secret of life, to develop new functional medicine for curing many difficult diseases and promote the development of information science. What we should do is to further penetrate the interaction between small molecules and nucleic acids and protein, and to develop rapid and convenient assays for nucleic acids and protein. Therefore, it is a hot point in bio-analytical chemical research.This thesis focused on the development of new probes of nucleic acids and proteins and to establish sensitive methods for the quantitative determination of them, used the fluorescence and resonance light scattering (RLS) as the primary technique. The absorption, determination of surface tension, electrophoresis by TV, TEM techniques were also used to study the interaction mechanism. In the first part of this thesis, we commented on the basic theory and progress of luminescent probes for nucleic acid and protein. The developing tendency of this area was also included in this part. In the other parts, four systems for the determination of nucleic acids and proteins.In the second section, we developed Eu³⁺-benzoylacetone (BA) complex as fluorescent probe to detect micro amounts of nucleic acids. The study indicated that in the presence of cetyltrimethyl-ammonium bromide (CTMAB) the instrinc fluorescence intensity of Eu³⁺ in Eu³⁺-benzoylacetone (BA) complex can be greatly...