Studies On Determination Of DNA By Resonance Light-scattering & Fluorescence Methods And Their Applications

AbstractBnd on a lot of documnts arid conditions of our ltw, tathesis edM seved new Won methch of DNA by means ofnovl co lightscatterin opS) asendque, and the boonmecbosms are invedpe by various speCtheWis thesis is compoho of four chaPters fIn the ho chaPter the RLS to of RDsahilin with DNA haV boStudied In PH mp of 10.3-1 1, the RLS of Rosahain is ghy enhantal byM due tO the leron of RoWon the ler of M M is aax ligh SCattering pe at 485nm, the enhantal intensfy of arS atWhich is Prowtonal tO the COnCentalon of DNA. The linear Iangr of theMon ed is Glop ll gh with a condallon coefficien of 0.9980. Thededon ledt is l4.20 ll gh. This methed is simple, mpid and has beaPPlied tO the dedon of DNA in nded saxnples with Satisforry ed.In the and wi we the tha the RLS of Crystal VoletyCV) is wiN by W in PH W of 9.2-l0.5. The enhantal inboity of RLS at5l2nm is wiOnal tO the concentalon of DNA fiDm 0 to 900 P gh. Thecormlation Mcient is 0.9950. The deteChon ledt is 5.02 ll gh. the methedhas aPPlied tO the Mon of M in W Samgles successfully. Thembo is invwt --y by arS ~ and UV mpCOmpe with Other methwh, thes methed has lop deedve wavelength,whch can deduce the intederence of baCkgrOund and increase the intenSity ofresonance ligh SCatterin.In the dri ChaPter we hav studied the RLS petIa of Blere withN In acid M of pH 2.G2.8, the RLS of Btw is ed enhantalby W. The M inbeity of RLS is propenional to the coneendonof oc at 308nm. The linear rmge of the calibndon tw is un ll ghwh a cormlallon coefficed of 0.9972. The detection drit is l9.92 ll gh. msM bo co aPPed tO the dedon of oc in ln1xed samplesIIlsuccessfully. Based on the UV spectra, PIES spectra and the reported paper, we infer that with the decreasing of the ratio of Berberine to DNA, the interaction between Berberine and DNA transforms from giving priority to aggregaUon into giving priority to intercalation.In the first section of the foith chapter, the RLS system of Brilliant Crystal Blue(BCB)?DNA have been studied. The P18 of BCB is greatly enhanced by DNA in pH range of 10.8-11.5. The enhanced intensity ofRLS at 347nm is proportional to the concentration of DNA. The linear range of the calibration graph is 80-1000 ii g扡. The correlation coefficient is 0.9962. The detection limit is 2330~ig/L. This method has been applied to the determination of DNA in mixed samples with satisfactory results.In the second section of the forth chapter, Fluorescence quenching of BCB by DNA can be employed to determinate DNA sensitively. In the optional pH range from 5.0 to 9.0, when excited at 28mm by ultraviolet, the fluorescence of BCB at 670nm will be quenched by 1~DNA quantitatively.The linear range of the calibration graph is 0.1 -2.Smg/L. The cormlation coefficient is 0.9964. The detection limit is 88.0~ gL. This method has large Stokes Shiil~, which can greatly deduce the interference of background and increase the sensitivity.In the thinl section of the forth chapter, The UV absorption, fluorescence and resonance light-scattering spectra of BCB桪NA system have been studied. In the investigation of fluorescence polarization, fluorescence quenching and the statue of BCB in aqueous, the results support that the interaction between BCB with DNA transforms from giving priority to aggregation into giving priority to intercalation with the decreasing of the ratio ofBCB to DNA.