| The paper corvers four aspects: 1) the advances of the research into Thellungiella halophila; 2) study of effects of salt treatmeant on growth and salt-tolerant mechanism; 3) study of the osmotica and the osmotic adjustment ability of Thellungiella halophila; 4) study of effects of salt treatmeant on growth, the system of scavenging reactive oxygen species and the ablility of antioxidation of Thellungiella halophila in its bulting stage.We reviewed and discussed the research advances on the halophyte Thellungiella halophila from the following five aspects: (1) biological characteristics of Thellungiella halophila; (2) epicuticular waxes of Thellungiella halophila; (3) the presentation of the idea that Thellungiella halophila becomes the model plant of salt-resistance in the study of molecular biology; (4) study of the basic physiology of hellungiella halophila; (5) the existing problems and future prospect. The emphasis was put on the comparison between Thellungiella halophila with Arabidopsis thaliana in the biological characteristics, epicuticular waxes and genetic material, especially on the necessity of Thellungiella halophila becoming the salt- resistance of model plant in the studies of molecular biology.The experiment was to test the indexes of the fresh and dry weight of whole plant, the relative water content of whole plant, the succlence degree of whole plant, the ratio of roots/shoots, the organic dry weight, the inorganic dry weight, the roots activites and the nitrate reductase activities. The results showed (1) the fresh and dry weight of whole plant, the relative water content of whole plant, the succlence degrees of whole plant, the ratio of roots/shoots and the organic were decreased; (2) the organic dry weight of shoots were decreased and the inorganic dry weight of shoots were increased, while the organic and inorganic dry weight of roots were opposite; (3) the roots activities were increased; (4) the lower salt concentration treatment made the nitrate reductase activities increase while the higher salt concentration treatment made them decrease, but they all were higher than that of control plants. The analysis the growth indexes of Thellungiella halophila indicated that Thellungiella halophila isn't salt-delution halophyte. The observation of Thellungiella halophila leaves' surface by scanning electron microscope (SEM) indicated that there is no salt-gland or bladder on the surface of Thellungiella halophila, so it isn't recretophyte. Possibly, its salt-tolerance mechanism is based on the metabalism of the basic physiology and biochemistry.Thellungiella halophila was treated with different salt concentration. The content of various organic and inorganic osmotica in the shoots and roots of Thellungiella halophila were determined respectively and their contributions to the osmotic adjustment were calculated. Na+, K+, Cl-, NO3- were the most important inorganic osmotica; and soluble sugar and organic acid were the most important organic osmotica. Na+ X-ray microanalysis indicated that vacuoles in the mesophyll cells of leaves are the main sites which accumulate toxic ions.Thellungiella halophila in its bulting stage was treated with different NaCl concentration, determining relevant indexes, including the growth amount and the height of single plant, the number of branches and leaves, the membrane leakage and the MDA content of stem leaves and roots, the superoxide anion (O2-) content of stem leaves, theactivities of superoxide dismutase and peroxidase (POD) and calatase (CAT). The result showed (1) 50mmol/L NaCl treatment benefited Thellungiella halophila growth ,but 100-400mmol/L NaCl inhibited Thellungiella halophila growth; (2) 50-100mmol/L NaCl treatment made membrane leakage change insignificantly compared with the control plants, the content of malondialdehyde and superoxide anion decreased compared with the control plants; while 200-400mmol/L NaCl treatment brought about the increase of membrane leakage, content of malondialdehyde and reactive oxygen species amount compared with the control... |
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