Study On 2-d Electrophoresis Of Total Protein And Its Antibacterial Or Other Main Function Of Sarcophaga Peregrina's Endochylema

The flesh fly is Characterized by chest with light gray, black stripes, abdomen light gray and dark gray grid pattern.The flesh fly is an in-depth exploitation and utilization of resources of reproducibility insects. Besides crust is the quality of raw insects chitosan, cytoplasm containing rich protein, and many kinds of active protein. This paper analyses the protein flax flies and antibacterial activity.Research content one, established the whole protein extraction experiment scheme of flesh fly. Using water, alcohol, alkali, salt, obtained respectively water-soluble protein, gliadin, globulin, alkali soluble protein and water soluble protein, and measured the protein content by Brandford method, including albumin, micromolecule peptide, gliadi, respectivelywere 41% ,33% and 3.98%. It be taken as elementary materials for the flesh fly's protein study.Research content two, the map of total cytoplasm protein was established by using two-dimensional electrophoresis. The fat, pigment and polysaccharids of the flesh fly in the cytoplasm for two-way electrophoresis interference problems were solved. The best draft was obtained. It laid a foundation for flesh fly cytoplasm proteomics research.Research content three, the antioxidant-hydroxyl ability of protein was identificated by using salicylic acid method .Its antioxidant data were obtained, albumin, and the clearance of immunoglobulin hydroxyl radicals can achieve 80%, and other protein components of hydroxyl clear also can reach more than 45%. With extraction method by using filter paper to explore the antimicrobial activity of protein, the antimicrobial activity of protein were discovered obviously . The evidence was provided that flesh fly could be utilized in the category of hairdressing, health care and animal protein source of natural food.