Study On The Detection Methods And Characterization Of Bioactive Of Phosphatase Activity By Using SBR System

Sludge biological activity of sewage biological treatment system is animportant index to analyze, appraise and forecast the operation conditions andthe system treatment ability. Among all the indexes for sludge biological activity,the electron transport system ALP activity has been paid more and more attentionall over the world for recent years. It can not only reflect the organic matterdegradation ability of microbe in terms of biology, but also judge the organicmatter degradation velocity and operation conditions of the treatment equipmentsin view of biological activity. The research and application of sludge ALPactivity detection technology is very important to accelerate the technologydevelopment of wastewater biological treatment and to enhance and improve theoperation control of a sewage biological treatment system.In this dissertation, feasibility of ALP activity for evaluating biologicalactivity was studied for the first time by using single organic matter, NH4+-N andNO3--N as substrate; changing rules and activity status of ALP activity duringSBR process under different operation conditions were also investigatedsystemically for the first time.The maximum absorption wavelength of ALP were590nm respectively.The mole absorption coefficient (ε590) of ALP was16441L/(mol·cm) in acetonesolution (85%). ALP activity increased as culture temperature ascended in therange of10~50℃. Generation velocity level of ALP maintained relatively high during the culture time from0to30min. Chemical reduction occurs to ALP atexorbitant pH value. ALP activity decreased as sludge concentration ascended athigh sludge concentration. ALP activity test needed to exclude dissolved oxygen.Acetone solution (85%) was the best extraction solvent in ALP activity detection.When using single organic matter, NH4+-N and NO3--N as substrate, ALPreflected the change of organic matter biological degradation, nitrification anddenitrification course and sensed the impact load imposed on the system in time.It showed that it is feasible to adopt ALP activity to evaluate sludge biologicalactivity. Results from sludge biological activity dynamics analyses verified thatthe growing velocity of heterotrophic bacteria was higher than that ofnitrobacteria.ALP activity was reducing continuously during the period of organicmatter degradation in a SBR system at normal temperature, and there was a piton the curve when COD is removed completely. ALP activity increased and thendecreased at the beginning of nitrification course, and was in a stable period atthe end of nitrification. During the denitrification course with agitating,ALPactivity increased at first, then decreased at the end of denitrification course, andthen trended stably. Operation conditions changes did not affect changing rulesof ALP activity obviously in the SBR system. The emergence time of thedistinction pits that indicated different reaction stages on the curve, and theaverage ALP activity values in different stages happened to change.