| The objective of this paper mainly studies the ray of apocynum composition of the material or fungus,The strains of apocynum degumming of selection and identification,The resistance mutations in screening and enzyme analysis,Microbial degumming process of Apocynum, And the fiber degummed of the fiber degumming analysis and performance analysis, The main research contents and conclusions are as follows:The chemical composition of apocynum is analyzed, Analyses of apocynum ketone compounds antibacterial activity of microorganism, using AGAR diffusion method for determining the antimicrobial activity. The concentration of1g·L-1apocynum ketone compounds on bacillus subtilis, escherichia coli, beer yeast, aspergillus antibacterial activity for the32.835,15.734,21.521,21.322IU·mg-1. Therefore, ketone compounds on apocynum gram-positive bacteria had a stronger inhibitory effect of gram-negative bacteria, weak inhibitory effect.In this lab saved for pectinase produce aspergillus fungus strains of bacteria, based on protoplast ultraviolet mutation breeding, selection andhashigh resistance to both for pectinase strains of bacteria in the screening and enzyme activity analysis. Bacteriostatic circle of Mutants Aspergillus niger-Apocynum-3(AS-A-3) is the1.276times of bacteria.To the strains of the microbial degumming process. In this experiment condition, the influence of aerobic microorganisms degumming apocynum are the major factors pH value, bath ratio, temperature, time, and bacteria come unglued quantity, etc. Through orthogonal experiment and determine the optimal number of process for8:pH,40℃, temperature for degumming time for24h, degumming in bath ratio, the initial flora amount for each100mL degum, adding1.5%liquid is1.5mL seed bacterium fluid. In the enzymatic degumming process flow and using the method of control pH derived degumming process improvement. Weightlessness rate can be stable in the left28%steady at20%.By electron microscopy analyses of microbial degummed and not degum, fiber and the fineness of fibers, the breaking strength and breaking elongation, hygroscopicity, put wet test results show that the microbial degum better keep the original features, use fiber in our laboratory strains on the screening of apocynum microbial degum basically achieved the goal and requirements. Come unglued. |
