Construction Of The Collagen/Resveratrol Scaffold And Its Application In Cartilage Repair

In this work, the water-solubility of resveratrol (RSV) was first synthesised bycoupling with polyacrylic acid (PAA, Mn=1000) via1,1’-carbonyldiimidazole (CDI).The resulted PAA-RSV showed much better water solubility than RSV. ThePAA-RSV still kept the ability to capture DPPH free radicals produced by H2O2.PAA-RSV and collagen were then cross-linked by EDC/NHS to constructcollagen/RSV scaffold. The compressive strength of collagen/RSV scaffold was about1.1MPa.The cell compatibility of hydrogel was evaluated by MTT method. The resultsshowed that the hydrogel can promote the proliferation of chondrocytes and BMSCs.Comparing with collagen scaffold, the collagen/RSV scaffold showed bettercompatibility. The chondrocytes can grow better on the collagen/RSV scaffolds andsecrete more glycosaminoglycans. The BMSCs and chondrocytes onto thecollagen/RSV scaffold exhibited a better viability than those on collagen hydrogelafter exposed to H2O2.The collagen and collagen/RSV scaffolds were transgrafted into the articularcartilage defects of rabbit, respectively. After12weeks, the recovered tissue wasevaluated. The collagen/RSV scaffoldshave demonstrated better recovery thancollagen scaffold. It is anticipated that this collagen/RSV scaffold is promising to beapplicable in in treatment of arthritis.