A Study On Preparation Of Functionality Microcapsules And Research Of Technics Of Separation And Purification Of Glutathione

Microcapsules are capable of protecting core material, sustained release, controlled release, changing the form of core material and screening its smell so that microcapsule technology has been widely used in such fields as medcine, food, cosmetic, pesticide, fertilizer and dye industry.To improve the stability of vitamin A palmitate, microcapsules for vitamin A palmitate are prepared by single coacervation combined with spray drying with polyaspartic acid and gelatin as wall material. The optimal parameters were as follows:the ratio of polyaspartic acid to gelatin1:1(W/W); the ratio of core material to wall material1:4(W/W);wall material concentration10%(W/V), emulsifier concentration1.5%; the ratio of absolute alcohol to water1:2(V/V).For spray drying, the inlet air temperature160℃, and outlet air temperature70℃;The temperature of emulsification38℃. More than84.3%of vitamin A palmitate remains through seven days’ accelerated test at60℃.Furthermore, Vitamin A palmitate converted from oil to powder is convenient to be reserved, transported and further processed, so that the application field is expanded. Glutathione (GSH; L-y-glutamyl-L-cysteinylglycine) is a kind of useful tripetide which is widely distributed in cells in a variety of species and it has a considerable number of important physiological functions. Up to now, GSH has been produced industrially on a large scale mainly by means of yeast fermentation. Now the separation and purification of GSH mainly makes use of Cu-Salt Method and Ion-Exchange Method. It has been reported that there are some kinds of homemade ion exchange resin in use of the separation and purification of GSH, but the relevant techniques are not straightly suitable for industrial application. The aim of the research is to search a homemade ion exchange resin in place of imported resin in order to reduce production cost. After comparing various ion exchange and adsorbent resins, the two of them, Model001x7ion exchange resin and Model DM-18macropore adsorbent resin, were selected in use of separating GSH by two-stepped chromatogram, and good experimental results are obtained.In this research a process of separation and purification of GSH was established which includes the following cell operations:extraction of GSH, pretreatment of extraction solution, ultra-filtration, condensation, ion exchange chromatography, condensation, macropore adsorbent resins chromatography and crystallization.The recovery of the whole process is51.06%, and the purity of GSH crystal is97.8%.This research is mainly focused on the processes of chromatography, since the former steps and the crystallization are routine. The conditions of ion exchange chromatography are as follows:to fill the column to55cm in height with the60-100meshed resin pretreated, then to pump the sample solution of pH3.0-3.2into the column; to wash the column with deionized water; at last to elute the column with HC1solution of the gradient from0.15mol/l to0.30mol/l, The recovery of the step is about94%. To obtain high purity GSH, Chromatography with macropore adsorbent resin was performed. The process of chromatography was that the concentrated eluate of pH3.0-3.2was percolated in a column packed with DM-18resin. GSH was then eluted with water, The recovery of the step is about92%.