Study On Antioxidation Synergistic Effects Of Lycopene And The Stability Of Lycopene Inclusion Tablets

As a kind of important functional carotene, lycopene is considered to be a potentantioxidant and a singlet oxygen quencher, which can significantly reduce theincidence of cardiovascular, cancer and other diseases, and have certain protectiveeffect on male infertility or Alzheimer’s disease. As a result, lycopene have been a hotarea in food and medicine research field. However, lycopene is not only insoluble inwater but also sensitive to light, heat and oxygen, which significantly limited itsapplication. Therefore, how to improve the stability and bioavailability of lycopenehave been the spotlights at home and abroad. Here lycopene was refined using themethod of saponification-recrystllization; and the synergistic effects of lycopene withseveral vegetable oils or other antioxidants were also investigated; the preparationtechniques of lycopene-HP-β-CD inclusion and the stability of lycopene crystal orinclusion tablets were studied. The main works are described as follow:The refined lycopene was obtained using saponification-recrystallization metodswith lycopene oleoresin as material, the optimum process conditions were as follow:lycopene oleoresin: potassium hydroxide=1:2.52(w/w), ultrasonic saponificationtemperature57℃，ultrasonic saponification time160min; and the procedure forrecrystallization is dissolve the lycopene obtained by saponification in ethyl acetate atthe temperature of70℃, cooling the solution and stand for6h at room temperature,and then the lycopene can be separation by crystallization, with the purity of lycopenecrystal more than98%. And then the stability of lycopene crystal under differentpreservation conditions were studied. The results show that it is more favorable topreserve the lycopene crystal under the condition of low temperature and aftervacuum filling nitrogen.The scavenging capacity of lycopene alone and the combination with fourdifferent effective oils, namely flax seed oil, tomato seed oil, white grape seed oil and red grape seed oil were measured using the2,2-diphenyl-1-picrylhydrazyl (DPPH)free radical. The results suggest that the combination of lycopene and four differentkinds of vegetable oil showed significant synergism at certain concentrations, in aconcentration-dependent manner. Most noticeably were grape seed oil combined withlycopene. After that, the scavenging capacities of lycopene, grape seed oil, vitamin Eand procyanidine, alone and in different combinations were measured by orthogonaldesign, taking the scavenging capacity of antioxidants on DPPH as the mainevaluation index to optimization the composite antioxidant formula. The best ratio ofcomposite antioxidants is: lycopene（5g/mL）；grape seed oil（0.1mg/mL）；VE(25g/mL)；procyanidine（3g/mL）.After a comparison study on the result of inclusion, lycopene and HP-β-CD waschosen and the technology of the inclusion was optimize using inclusion rate asindexes through trituration method. Namely ration of materia1:50，temperature40℃，time1h. The lycopene content of the inclusion complex and inclusion rate at theoptimizing conditions reached1.67％(w/w) and83.71％(w/w), respectrively. Theresults showed that lycopene solubility of the inclusion were0.49mg/mL, then theresulted products were characterized by UV-vis spectroscopy and scanning electronmicroscopy. In addition, the stability of lycopene inclusion tablets under differentlight, temperature was also investigated.