Study On Anti-fatigue Activity Of Alkaloids Extract From Seeds Of Abelmoschus Esculentus (L.) Moench

Alkaloids were isolated and determined from the seeds of Abelmoschus esculentus (L.)Moench. The crude extract was texted for its anti-fatigue activity. Results from theseexperiments not only reveal the anti-fatigue effects of the alkoids components, but also lay thefoundation for the development of new products from the seeds of Abelmoschus esculentus (L.)Moench.The alkaloids from the seeds of Abelmoschus esculentus (L.) Moench. were extracted byethanol, and determined by color reactions and thin-layer chromatography (TLC). The methodof determining the content of caffeine (one important alkaloid in the seeds of Abelmoschusesculentus (L.) Moench) was employed by HPLC, through linear range, precision inspection,investigation reproducibility, stability study and recoveries. The mice were divided into fourgroups randomly,10mice per groups, they are alkaloids extract high-dose group, alkaloidsextract middle-dose group, alkaloids extract low-dose group, and the blank control group. Allof the mice in four groups are no significant defference by t-test. Mice in each group weregiven different doses of the test substance as following (by body weight): high-dose group oraladministrated alkaloid extract8mL/kg; middle-dose group oral administrated alkaloid extract4mL/kg; low-dose group oral administrated alkaloid extract2mL/kg; blank control group withdistilled water8mL/kg. All groups were oral administred the extrat every day for30d.Determine the change in swimming time, body weight, serum urea nitrogen, hemoglobin,blood lactate mice, and liver glycogen of each mouse, respectively. The test data werestatisticed by SPSS21.0, and analysiz of the significance by analysis of variance.We got crude extract4.280g from1.325kg seeds of Abelmoschus esculentus (L.) Moench.Alkoids were identified by color reaction and TLC. The content of caffeine determined byHPLC, the RSD of precision, stability, recovery, were0.09%,0.65%and1.82%, respectly. Theaverage recovery was99.43%. Weight-bearing physical swimming time in all of the oraladministrated alkaloid extract groups. And high-dose group coumpared with control group.After swimming, in high and middle-dose group, serum urea nitrogen was significantlylower than control group; there was significant difference beteewn high-dose group andcontrol-dose group in every detection. Different doses of alkaloids extract had different effectson serum urea nitrogen in swimming mice (P <0.01). There was significant difference (P<0.01)beteewn high-dose group and control group. Different doses of alkaloids extract have different effects on hemoglobin in swimming mice (P <0.01), and presents a clear dose relationship.Blood lactate levels of mice in high-dose and middle-dose group were significantly lowerthan control group (P <0.01). Compared to the control group, the high-dose group had the mostsignificant difference (P <0.01). Different doses of alkaloids extract had different effects onblood lactate in swimming mice (P <0.01).After swimming, glycogen content in the three different dose groups were significantlyhigher than the control group (P <0.05). Compared to the control group, the high-dose grouphad the most significant difference (P <0.01). Different doses of alkaloids extract had differenteffects on glycogen content in swimming mice.