Study On The Fabrication And Application Of Glass Micropipette

With the development of science and technology, glass micropipettes have been applied in biological fields, for example, cell microinjection technology.This paper mainly studies the fabrication and structural optimization of the quartz glass micropipette. And the applications in biological microelectrode and neutron penumbral imaging technology have been investigated.The glass micropipettes are fabricated in the laser micropipette puller(P-2000) based on orthogonal experiment method to optimize the process parameters. The final optimum parameters are with the Heat setting of 700, the Velocity setting of 45, the Delay setting of 145 and the Pull setting of 225. Then after the process of cutting and grinding, we get the micropipettes with a tip diameter of 10μm, and the change value of the tip diameter located at the length of 100μm is as small as 11.34μm. Then its application for functional electrical stimulation of muscle tissue is investigated and the corresponding experiments are performed. A bipolar functional electrical stimulation signals of 1mA, duration 50 ms are delivered by the implanted microelectrodes to obtain the Electromyography of rana catesbeiana.This paper describes the fabrication of thin film carbon electrodes with special shape. The orthogonal experiments are performed to optimize the process parameters of micropipettes. The experimental results show that the optimum parameters are with the Heat of 700, the Velocity of 40, the Delay of 140 and the Pull of 250. Then we get the micro-rods with a tip diameter of 10μm, and the change value of the tip diameter located at the length of 100μm is as small as 11.01μm after grinding. Then, a 5μm layer of parylene C is deposited on micro-rod by CVD process. Parylene films are pyrolyzed in a furnace at 900℃ for 1 hour under an inert N2 atmosphere, which converts the highly insulating parylene layer into a conductive carbon film. Finally this paper examines the electrochemical experiments are performed by three carbon glass electrodes under cyclic voltammetry.This paper also introduces the Monte Carlo method and operation method of MCNP software. The simulation calculation of ideal double cone penumbra hole is presented base on the material of W and the Point Spread Function(PSF)is very good. However, the micropipette hole PSF of Cu、Pb and Au is not good.