Electrochemical Behavior Of Hemoglobin At A Lauric Acide Modified Glassy Carbon Electrode And Analysis Of The Data

As we all know that almost all the chemical reactions forming the organism metastasis are processing under the active protein -methane. The most foundational movement in animal body is the charge's .The basis subject of biology science also includes electrochemistry. Therefore, electrochemical methods are one of the beneficial tools exploring the biological proud mystery. Hemoglobin is an important sort of biological macromolecular in human body and substantial basis of biological activity. Hb's quantitative analysis is a significant index in application analysis, biological analysis and disease examination and the research of its electrochemistry behavior.From the aspect of data processing, how to acquire the process information according to the experimental data and observe the inner evolution laws is the subject paid attention to by all potential research fields.Electrochemistry behavior of Hb on GC electrode modified by a Lauric Acid was researched, which data was analyzed in details. The main work and corresponding conclusions are outlined as follow:1. The character of Hb on a Lauric Acid modified GC electrode was analyzed by the method of CV, which results show that the electrochemical deoxidization of Hb on modified electrode was an irreversible charge transfer system. i_p increased with the increasing concentration of Hb and the value of pH . When scan rate was increased , the value of Ep was more negative.2. According to the quantitative analysis of Hb's i_p and v, the results indicate that it is difficult to predicate which is the dominant function between adsorption and diffusion. However, maybe they react together.3. A treatment of the peak current i_p involving adsorbed and diffusedreactants has been worked out with the aid of computer simulation. When a simulated data set is generated, the errors added to every theoretical data point are all generated at random between -2 to +2% of the theoretical values.The theoretical data set [ ip,v ] can be constructed according to / = kd xv1'2 +kaxv, then, respectively they were dealt with data linear recursive by ipto v1/2 and ipto v, which results were similar with theformer conclusion. Furthermore, the conclusion of adsorption and diffusion reacting together was approved.According to processing the experimental data under different pH by means of Genetic algorithms and Gauss-Newton algorithms, then, we can get the relation between simulation parameter and the value of pH: simulation parameter kd increased by linear with the value of pH.kd =-3.779xl(T5+1.109xl(r5x/>//It means that the mechanism which pH of the solution impacts the diffusion of Hb in electrolyte is simple;Simulation parameter ka increases by parabola with the value of pH. ka =2.The equation shows that when the value of pH is 6.352, the absorbance of Hb on a Laurie Acid modified GC electrode is the least.4. Because of the adsorption action of electrochemical active substance, the Michaelis-Menten model can not reflect the inner relationship between C and ip. Then, when a term, which had contribution to i was gone intothe Michaelis-Menten equation, the applicability of the new model was increased.5. After analyzing the cyclic voltammograms and the experimental data, The reaction mechanism of Hb on modified electrode was outlined as follows:Hb heme(Felll) + e CH3(CH)i0COOH Hbheme(Fell)-------------------------------?...