Isolation, Identification And Bioactivity Investigation Of The Water-soluble Chemical Constituents Of Tetraena Mongolica Maxim

Tetraena mongolica Maxim (T. mongolica), growing in salinized desert, is a member of Zygophyllaceae. It was regarded as a living fossil and flora panda owing to its ancient origin and endangerment. It is reported that desert plants contain many metabolites because of harsh growing conditions, however, the chemical constitutes reported in T.mongolica were limited. Further researches are required to reveal its unique metabolism products and functions. This study was carried out to investigate the water-soluble components of T. mongolica.Firstly, the plant material was extracted with methanol under reflux; then, the extracts were suspended in water and partitioned with petroleum ether, ethyl acetate, and butyl alcohol; the H2O soluble part was subjected to different chromatographys to obtain pure compounds. These compounds were identified by analysis of their NMR, ultraviolet, infrared and high resolution mass spectra. In addition, the biological activities and quantification were inestigated. And we schemed the possible biosynthesis pathway of compound 3. The results of this study are as follows: 1. Four compounds including three new compounds and one compound firstly reported from nature were isolated from T. mongolica and their structures deterned by spectroscopic methods. Compound 3 was shown to contain an unprecedented heterocyclic skeleton with nitrogen-oxygen-nitrogen bonds. Compouds 1-3 contain a sulfate group in their structure.2. UPLC-MS was used for the quantification of the constituents. The result showed that the content of these constituents was about 4.71-116.15 μg/g. And we assumed the possible mechanism for the transformation of 2 into 3.3. None of the four isolated compounds showed cytotoxicity on HepG2 cells. These compound were also tested for their protective effects on human hepatoma HepG2 against tert-butyl hydroperoxide (t-BOOH) induced oxidative stress. Compounds 2 and 4 showed significant protective effects on cellular oxidative stress.