Studies On Development Of Somatic Embryo Of Tetraena Mongolica Maxim

Development of Somatic embryo of Tetraena mongolica a endangered rare plant growing in the desert areas of Inner Monglia was studied by using the techniques of solid culture and suspension culture. The results Showed that: (1)The best medium for the development of somatic embryo of Tetraena mongolica was MS added 2,4-D (O.lmgL~?and 6-BA (0.25 mg U?; (2) Callus of Tetraena mongolica produced from the continuous fission of the cells of explant like cotyledon the cells embronal axis, cambial initial cells of root , phloem cells and bundle sheath cell . The initial stem of organ in callus belonged to outside origin and the root was the style of inside origin; (3) Somatic embryo development was related to the starch grain cumulation . The starch grain disixibuted closely around ball like embryo the shoot would oppear from,so the starch grain was a active metabolizable matter for the organ formation in the course of tissue culture; (4) Somatic embryo of Tetraena rnonglica developed from single cell of I, type in vigorous fission under suspension culture. The developing process of somatic embryo was similar to the development of Tetraena tnonglica. The main factor affected somatic embryo development of I, type was subinoculation of callus and the type as well as the proportion of hormones; (5) Vigor change of SOD was related to the formation of callus and somatic embryo development process of Tetraena mongolica.