| Rice is not only a mode plant in monocotyledon but also one of the most important gramineous grain crops in the world. The achievement of the rice genome is the basic for the studies of the rice gene functions. In this text, we screened rice mutants from a T-DNA insertion pool. The analyses of the mutant phenotypes and gene cloning were progressed.A high straw mutant 367 was screened from the T-DNA insertion mutant pool. Compared to wild-type in young seedling,367 mutant displayed increased number of primary roots, length of leaves, and number of lateral veins. But the 367 plant was only a little higher than wild-type. When developing to adult plants,367 was obviously increased the length of eustipes, the number of tillows and the size of seeds. However, the seed setting rate and fertility of 367 were reduced. With the observation of the leaf epidermis using dental resin impressions, we found that the stomatal density and number of epidermal cells in unit area were increased. Phenotypic and genetic analyses of 367 indicated that the high straw phenotype can be stably inherited. At present, we are cloning the gene of 367 by the hiTAIL-PCR technique.Agrobacterium-mediated transgene technique makes it possible that the related rice gene and its homologous gene of Arabidopsis can be overexpressed or interfered in different mode plants to further understanding the function of this gene. In this study, we overexpressed OsPIPE, the Arabidopsis PIPE homologous gene in rice, to pipe mutant in Arabidopsis by Agrobacterium-mediated transgene technique. We found that the OsPIPE could rescue the stem length and anomalistic curled leaves of pipe. The length of lobe and neck and pavement cell shape in 3th and 4th leaf of pipe was also rescued. Then, the OsPIPE also was knocked out in rice by RNAi. The transgenic plants showed similar dwarfed phenotype. All results suggest that the function of the OsPIPE gene is similar to that of Arabidopsis in regulating plant development. |
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