Map-basedcloningand Analysis Of Fluorescence Gene E61-2 Inarabidopsis Thaliana

Photosynthesis is the one of the most important physiological activities for green plants. Chloroplast is the organelle where photosynthesis carries on in green plants. In chloroplast, a variety of pigments are involved in photosynthesis, and are named photosynthetic pigments. Such as chlorophyll a. Chlorophyll in plants are fluorescent probesinvolving a wide range of applicase that enables the measurement fast and simple, non-invasive plants, etc. Most of measuring work about plant photosynthesis reaction and regulation information. Any factors that affect the environment, such as temperature, light, moisture, salinity, mineral elements, CO2 concentration, hormones, can be used to change the parameters of the reflected fluorescence nutrition, etc, causing impaired chloroplast development. Based on plant chlorophyll fluorescence characteristics and in-depth study of the theory of photosynthesis, chlorophyll fluorescence kinetics technology has been rapid development, Chlorophyll fluorescence kinetics of theoretical research into production of chlorophyll fluorescence imager is photosynthetic characteristics of our study and is the strong and powerful tool. On the basis of the study, chlorophyll fluorescence probe has been widely used.In this study, the WT(Arabidopsis wild-type Col-0) is used as the experimental background, and is used as methyl sulfonic acid ethyl ester(ethylmethyl sulfonate, EMS).All WT seeds were through using chlorophyll fluorescence imaging screening. Chlorophyll fluorescence parameter value of mutant is less than the WT. The mutant phenotype can stable heredity, and it is named e61-2. Genetic analysis found that the e61-2 phenotypes comply with recessive single-base genetic. Crossing mutant e61-2 with wild typeLansberg(Ler), using the method of map-based cloning mutation hybrid F2 generation,the mutant is focused to point in the downstream of arabidopsis chromosome 1.Will useing the map-based cloning technology, thegene will be located in the mutation eventually T9L24 BAC, with the exchange rate 0.309%. Through genetic and analysis, that the mutant gene mutation may have links with chlorophyll synthesis. It is required for further research and analysis.