The Effect Of Aluminum Toxicity On Plant Growth And Aluminnum Related Gene Function Analysis

Plants may be subjected to kinds of abiotic stresses in the process of growth. Aluminum toxicity and low pH is a major environmental factor limiting plant growth and production on acid soils and50%of the world’s arable land is under the threat of soil acidification. Aluminum toxicity may be triggered in both acid and alkaline conditions due to the complex chemistry of Al. The trivalent Al species (Al3+) dominates in acid conditions (pH<5.5), whereas Al (OH)4-in alkaline conditions and an extremely toxic polynuclear Al species, Al)3can also form when Al solutions are partially neutralized with a strong base. In recent years, the researchers cloned a series of genes associated with aluminum toxicity. At the molecular level there has a certain explanation how plant response the aluminum toxicity, however the main stress mechanism of aluminum toxicity in plant is still unknown. Puccinellia tenuiflora, P. tenuiflora of gramineous, also known as the star grass, it is a perennial herbs that mainly distributed in saline-alkali land in the northeast China plain. Because of its strong adaptability, P. tenuiflora can grow in the extremely severe salinization of soil, and even in pH up to11of the soil it can also be normal growth. P. tenuiflora is important to restore the saline soil vegetation, but at present the research on mechanism of aluminum toxicity in P. tenuiflora was not reported. In this work, we observe P. tenuiflora and rice in aluminum toxicity and pH, use SEM observe P. tenuiflora leaf and root, we screened PutSTE24gene under aluminum stress to predict its subcellular localization of proteins by using bioinformatics analysis software, and the result showed that this gene was in the endoplasmic reticulum, which was the same with expressing PutSTE24gene in yeast INVsI. Under aluminum and saline-alkali stress conditions, the relative mRNA expression levels of the PutSTE24of alkaline grass were measured by real-time quantitative PCR, and the results demonstrated that its expression was induced up regulation. We also constructed a plant expression vector of PutSTE24gene, and then transferred into Arabidopsis. For further determine the correlation between PutSTE24gene and aluminum stress, we choose transgenic seeds for resistance experiment, which provided a wealth of theoretical and experimental basis for further research on interaction of related genes and regulatory mechanism of aluminum stress.